Novel Poly(urethane-aminoamides): An in vitro study of the interaction with heparin

P. Petrini, M. C. Tanzi, L. Visai, F. Casolini, P. Speziale

Research output: Contribution to journalArticle

Abstract

In order to obtain heparin-binding polyurethanes, tertiary amino-groups have been introduced in the polymer backbone by attributing a key-role to the chain extender, i.e. substituting butanediol, commonly used in polyurethane synthesis, with a tailor-made diamino-diamide-diol. In this work a poly(ether-urethane-aminoamide) (PEU/PIME/al) was obtained with poly(oxytetramethylene) glycol 2000, 1,6-hexamethylene-diisocyanate and the new chain extender, in the molar ratio 1:2:1. The heparin binding capacity of PEU/PIME/al was evaluated with 125I labelled heparin, using for comparison the analogous polymer obtained with a diamide-diol (i.e. the poly(ether-urethane-amide) PEU/PIBLO/al), and two commercially available biomedical polyurethanes (Pellethane 2363 and Corethane). pH and ionic strength dependence of the heparin uptake were investigated by treating all the polyurethanes with solutions of 125I heparin into buffers from pH 4 to 9 or NaCl molarity from 0.0 to 1.0. The stability of the interaction with bound heparin was investigated by sequential washing treatments (PBS, 1 N NaOH, 2% SDS solution), then analysing the residual radioactivity on the materials. Results indicated that the heparin binding of PEU/PIME/al is significantly higher and more stable than that of the other polyurethanes, with a time-dependent kinetic. The interaction with heparin appears to be prevalently ionic, with the contribution of other electrostatic and hydrophobic interactions. Activated partial thromboplastin time (APTT), performed on human plasma with polyurethane-coated, heparinized test tubes, indicated that bound heparin maintains its biological activity after the adsorption.

Original languageEnglish
Pages (from-to)353-365
Number of pages13
JournalJournal of Biomaterials Science, Polymer Edition
Volume11
Issue number4
Publication statusPublished - 2000

Keywords

  • Coagulation tests
  • Heparin adsorption
  • Heparin release
  • Poly(urethane-amides)
  • Radiolabelled heparin
  • Segmented polyurethanes

ASJC Scopus subject areas

  • Biophysics

Fingerprint Dive into the research topics of 'Novel Poly(urethane-aminoamides): An in vitro study of the interaction with heparin'. Together they form a unique fingerprint.

  • Cite this