Novel rat calpastatin mRNA variants

A. Servadio, G. Casari

Research output: Contribution to journalArticle

Abstract

Rat calpastatin cDNAs obtained by RT-PCR method were isolated and directly sequenced, this allowed the identification of two new variants both characterized by a 23 amino acids deletion at the end of the unique N-terminal domain L, increasing the number of functional isoforms. The deletion shows high homology to the amino acid sequence coded by exon 8 of human calpastatin gene and properly conserved splicing consensus sequences, suggesting exon skipping in domain L. Furthermore, three point mutations scattered along the coding region were found.

Original languageEnglish
Pages (from-to)721-725
Number of pages5
JournalBiochemistry and Molecular Biology International
Volume30
Issue number4
Publication statusPublished - 1993

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Rats
Exons
Amino Acids
Amino Acid Sequence Homology
Messenger RNA
Consensus Sequence
Point Mutation
Protein Isoforms
Complementary DNA
Genes
Polymerase Chain Reaction
calpastatin

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Novel rat calpastatin mRNA variants. / Servadio, A.; Casari, G.

In: Biochemistry and Molecular Biology International, Vol. 30, No. 4, 1993, p. 721-725.

Research output: Contribution to journalArticle

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