Abstract
Rat calpastatin cDNAs obtained by RT-PCR method were isolated and directly sequenced, this allowed the identification of two new variants both characterized by a 23 amino acids deletion at the end of the unique N-terminal domain L, increasing the number of functional isoforms. The deletion shows high homology to the amino acid sequence coded by exon 8 of human calpastatin gene and properly conserved splicing consensus sequences, suggesting exon skipping in domain L. Furthermore, three point mutations scattered along the coding region were found.
Original language | English |
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Pages (from-to) | 721-725 |
Number of pages | 5 |
Journal | Biochemistry and Molecular Biology International |
Volume | 30 |
Issue number | 4 |
Publication status | Published - 1993 |
ASJC Scopus subject areas
- Biochemistry
- Genetics
- Molecular Biology