Novel spastin mutations and their expression analysis in two Italian families

Annamaria Molon, Pasquale Montagna, Corrado Angelini, Elena Pegoraro

Research output: Contribution to journalArticlepeer-review


Mutations in spastin cause the most common form of pure autosomal dominant hereditary spastic paraparesis (SPG4). Here, we report two Italian families affected with SPG4-linked HSP harboring two novel spastin mutations. SSCP/sequencing analysis of the spastin gene showed a single base pair deletion causing a frame-shift in one family (1442deIT) and a missense mutation (1726T>C) resulting in a leucine to proline amino-acid change (L534P) in the other family. Total RNA from the mutant and the wild-type spastin allele in muscle biopsies from patients from the two affected families was quantitated. RNA expression was almost absent from the spastin allele harboring the single base pair deletion, while it was nearly normal for the spastin allele harboring the missense mutation. These data suggest that varying spastin RNA levels are found in out-of-frame and missense spastin mutations and imply different mechanisms involved in the molecular pathology of SPG4 linked HSP.

Original languageEnglish
Pages (from-to)710-713
Number of pages4
JournalEuropean Journal of Human Genetics
Issue number9
Publication statusPublished - Sep 1 2003


  • Dominance
  • Spastic paraparesis
  • Spastin
  • SPG4

ASJC Scopus subject areas

  • Genetics(clinical)


Dive into the research topics of 'Novel spastin mutations and their expression analysis in two Italian families'. Together they form a unique fingerprint.

Cite this