Novelty-related behavior of young and adult dopamine transporter knockout rats: Implication for cognitive and emotional phenotypic patterns

A. Adinolfi, C. Carbone, D. Leo, R. R. Gainetdinov, G. Laviola, W. Adriani

Research output: Contribution to journalArticle

Abstract

Attention deficit hyperactivity disorder (ADHD) is a neuropsychiatric disorder characterized by a developmentally inappropriate, pervasive and persistent pattern of severe inattention, hyperactivity and impulsivity. Despite onset in early childhood, ADHD may continue into adulthood with substantial impairment in social, academic and occupational functioning. A new animal model of this disorder was developed in rats with genetic deletion of the dopamine transporter (DAT) gene (dopamine transporter knockout rats; DAT-KO rats). We analyzed the behavior of DAT-KO rats for a deeper phenotypical characterization of this model. We first tested rats of the 3 genotypes at different ages (preadolescent, adolescent and adult), in a novelty-seeking test using a black/white box (Experiment 1). After that, we tested adult rats in a novelty-preference test using a 3-chamber apparatus with different shapes (Experiment 2). Experiment 1: as evidenced by analysis of time spent in the novel environment, adult DAT heterozygous (DAT-HET) rats show an increased curiosity-driven exploration compared with wild-type (WT) controls while DAT-KO rats did not recognize novelty. The locomotor activity data show a minimal difference between genotypes at adolescent age while the preadolescent and adult DAT-KO rats have significantly increased activity rate compared with WT and DAT-HET subjects. Experiment 2: in this case, due to more clearly evident spatial differences, time spent in novel environment was not significantly different among genotypes. During first 10 minutes, DAT-KO rats showed a decreased hyperactivity, apparently related to curiosity and attention to the new environments. In conclusion, DAT-KO rats may show some inattention while more novelty-seeking traits appear in DAT-HET rats.

Original languageEnglish
Article numbere12463
JournalGenes, Brain and Behavior
Volume17
Issue number4
DOIs
Publication statusPublished - Apr 1 2018

Fingerprint

Dopamine Plasma Membrane Transport Proteins
Young Adult
Exploratory Behavior
Genotype
Attention Deficit Disorder with Hyperactivity
Impulsive Behavior
Locomotion
Animal Models

Keywords

  • ADHD
  • adolescence
  • DAT-HET (heterozygous)
  • explorative curiosity
  • hippocampus
  • inattention
  • juvenile
  • novelty preference
  • novelty seeking
  • prefrontal cortex

ASJC Scopus subject areas

  • Genetics
  • Neurology
  • Behavioral Neuroscience

Cite this

Novelty-related behavior of young and adult dopamine transporter knockout rats : Implication for cognitive and emotional phenotypic patterns. / Adinolfi, A.; Carbone, C.; Leo, D.; Gainetdinov, R. R.; Laviola, G.; Adriani, W.

In: Genes, Brain and Behavior, Vol. 17, No. 4, e12463, 01.04.2018.

Research output: Contribution to journalArticle

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