NqA: An R-based algorithm for the normalization and analysis of microRNA quantitative real-time polymerase chain reaction data

Paolo Verderio, Stefano Bottelli, Chiara Maura Ciniselli, Marco Alessandro Pierotti, Manuela Gariboldi, Sara Pizzamiglio

Research output: Contribution to journalArticle

Abstract

In this note, we propose an R function named NqA (Normalization qPCR Array, where qPCR is quantitative real-time polymerase chain reaction) suitable for the identification of a set of microRNAs (miRNAs) to be used for data normalization in view of subsequent validation studies with qPCR data. NqA is available through the website of the Fondazione IRCCS Istituto Nazionale dei Tumori of Milan (http://www.istitutotumori.mi.it/modules.php?name= Content&pa=showpage&pid=812) with a dedicated user's guide. We applied our function on a qPCR dataset downloaded from the Gene Expression Omnibus (GEO) database. Results show that NqA provides a functional subset of reference miRNAs and a set of promising significantly modulated miRNAs for subsequent validation studies.

Original languageEnglish
Pages (from-to)7-9
Number of pages3
JournalAnalytical Biochemistry
Volume461
DOIs
Publication statusPublished - Sep 15 2014

Keywords

  • Algorithm
  • microRNA
  • Normalization
  • qPCR
  • R package
  • Software

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Medicine(all)

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