Nuclear grade and DNA ploidy in stage IV breast cancer with only visceral metastases at initial diagnosis

Mario De Lena, Anna Barletta, Francesco Marzullo, Mario Rabinovich, Bernardo Leone, Carlos Vallejo, Mario Machiavelli, Alberto Romero, Juan Perez, Juan Lacava, Maria Andrea Cuevas, Ricardo Rodriguez, Francesco Schittulli, Angelo Paradiso

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aims and background: The presence of early metastases to distant sites in breast cancer patients is an infrequent event whose mechanisms are still not clear. The aim of this study was to evaluate the biologic and clinical role of DNA ploidy and cell nuclear grade of primary tumors in the metastatic process of a series of stage IV previously untreated breast cancer patients with only visceral metastases. Methods: DNA flow cytometry analysis on paraffin-embedded material and cell nuclear grading of primary tumors was performed on a series of 50 breast cancer patients with only visceral metastases at the time of initial diagnosis. Results: Aneuploidy was found in 28/46 (61%) of evaluable cases and was independent of site of involvement, clinical response, time to progression and overall survival of patients. Of the 46 cases evaluable for nuclear grade, 5 (11%), 16 (35%) and 25 (54%) were classified as G1 (well-differentiated) G2 and G3, respectively. Nuclear grade also was unrelated to response to therapy and overall survival, whereas time to progression was significantly longer in G1-2 than G3 tumors with the logrank test (P <0.03) and multivariate analysis. Conclusions: Our results seem to stress the difficulty to individualize different prognostic subsets from a series of breast cancer patients with only visceral metastases at initial diagnosis according to DNA flow cytometry and nuclear grade.

Original languageEnglish
Pages (from-to)386-389
Number of pages4
JournalTumori
Volume82
Issue number4
Publication statusPublished - Jul 1996

Fingerprint

Ploidies
Breast Neoplasms
Neoplasm Metastasis
DNA
Flow Cytometry
Survival
Neoplasm Grading
Aneuploidy
Paraffin
Reaction Time
Neoplasms
Multivariate Analysis

Keywords

  • breast cancer
  • nuclear grade
  • ploidy
  • visceral metastases

ASJC Scopus subject areas

  • Cancer Research

Cite this

De Lena, M., Barletta, A., Marzullo, F., Rabinovich, M., Leone, B., Vallejo, C., ... Paradiso, A. (1996). Nuclear grade and DNA ploidy in stage IV breast cancer with only visceral metastases at initial diagnosis. Tumori, 82(4), 386-389.

Nuclear grade and DNA ploidy in stage IV breast cancer with only visceral metastases at initial diagnosis. / De Lena, Mario; Barletta, Anna; Marzullo, Francesco; Rabinovich, Mario; Leone, Bernardo; Vallejo, Carlos; Machiavelli, Mario; Romero, Alberto; Perez, Juan; Lacava, Juan; Cuevas, Maria Andrea; Rodriguez, Ricardo; Schittulli, Francesco; Paradiso, Angelo.

In: Tumori, Vol. 82, No. 4, 07.1996, p. 386-389.

Research output: Contribution to journalArticle

De Lena, M, Barletta, A, Marzullo, F, Rabinovich, M, Leone, B, Vallejo, C, Machiavelli, M, Romero, A, Perez, J, Lacava, J, Cuevas, MA, Rodriguez, R, Schittulli, F & Paradiso, A 1996, 'Nuclear grade and DNA ploidy in stage IV breast cancer with only visceral metastases at initial diagnosis', Tumori, vol. 82, no. 4, pp. 386-389.
De Lena M, Barletta A, Marzullo F, Rabinovich M, Leone B, Vallejo C et al. Nuclear grade and DNA ploidy in stage IV breast cancer with only visceral metastases at initial diagnosis. Tumori. 1996 Jul;82(4):386-389.
De Lena, Mario ; Barletta, Anna ; Marzullo, Francesco ; Rabinovich, Mario ; Leone, Bernardo ; Vallejo, Carlos ; Machiavelli, Mario ; Romero, Alberto ; Perez, Juan ; Lacava, Juan ; Cuevas, Maria Andrea ; Rodriguez, Ricardo ; Schittulli, Francesco ; Paradiso, Angelo. / Nuclear grade and DNA ploidy in stage IV breast cancer with only visceral metastases at initial diagnosis. In: Tumori. 1996 ; Vol. 82, No. 4. pp. 386-389.
@article{01788abde20d49ba8cb6b9902da289ec,
title = "Nuclear grade and DNA ploidy in stage IV breast cancer with only visceral metastases at initial diagnosis",
abstract = "Aims and background: The presence of early metastases to distant sites in breast cancer patients is an infrequent event whose mechanisms are still not clear. The aim of this study was to evaluate the biologic and clinical role of DNA ploidy and cell nuclear grade of primary tumors in the metastatic process of a series of stage IV previously untreated breast cancer patients with only visceral metastases. Methods: DNA flow cytometry analysis on paraffin-embedded material and cell nuclear grading of primary tumors was performed on a series of 50 breast cancer patients with only visceral metastases at the time of initial diagnosis. Results: Aneuploidy was found in 28/46 (61{\%}) of evaluable cases and was independent of site of involvement, clinical response, time to progression and overall survival of patients. Of the 46 cases evaluable for nuclear grade, 5 (11{\%}), 16 (35{\%}) and 25 (54{\%}) were classified as G1 (well-differentiated) G2 and G3, respectively. Nuclear grade also was unrelated to response to therapy and overall survival, whereas time to progression was significantly longer in G1-2 than G3 tumors with the logrank test (P <0.03) and multivariate analysis. Conclusions: Our results seem to stress the difficulty to individualize different prognostic subsets from a series of breast cancer patients with only visceral metastases at initial diagnosis according to DNA flow cytometry and nuclear grade.",
keywords = "breast cancer, nuclear grade, ploidy, visceral metastases",
author = "{De Lena}, Mario and Anna Barletta and Francesco Marzullo and Mario Rabinovich and Bernardo Leone and Carlos Vallejo and Mario Machiavelli and Alberto Romero and Juan Perez and Juan Lacava and Cuevas, {Maria Andrea} and Ricardo Rodriguez and Francesco Schittulli and Angelo Paradiso",
year = "1996",
month = "7",
language = "English",
volume = "82",
pages = "386--389",
journal = "Tumori",
issn = "0300-8916",
publisher = "SAGE Publications Ltd",
number = "4",

}

TY - JOUR

T1 - Nuclear grade and DNA ploidy in stage IV breast cancer with only visceral metastases at initial diagnosis

AU - De Lena, Mario

AU - Barletta, Anna

AU - Marzullo, Francesco

AU - Rabinovich, Mario

AU - Leone, Bernardo

AU - Vallejo, Carlos

AU - Machiavelli, Mario

AU - Romero, Alberto

AU - Perez, Juan

AU - Lacava, Juan

AU - Cuevas, Maria Andrea

AU - Rodriguez, Ricardo

AU - Schittulli, Francesco

AU - Paradiso, Angelo

PY - 1996/7

Y1 - 1996/7

N2 - Aims and background: The presence of early metastases to distant sites in breast cancer patients is an infrequent event whose mechanisms are still not clear. The aim of this study was to evaluate the biologic and clinical role of DNA ploidy and cell nuclear grade of primary tumors in the metastatic process of a series of stage IV previously untreated breast cancer patients with only visceral metastases. Methods: DNA flow cytometry analysis on paraffin-embedded material and cell nuclear grading of primary tumors was performed on a series of 50 breast cancer patients with only visceral metastases at the time of initial diagnosis. Results: Aneuploidy was found in 28/46 (61%) of evaluable cases and was independent of site of involvement, clinical response, time to progression and overall survival of patients. Of the 46 cases evaluable for nuclear grade, 5 (11%), 16 (35%) and 25 (54%) were classified as G1 (well-differentiated) G2 and G3, respectively. Nuclear grade also was unrelated to response to therapy and overall survival, whereas time to progression was significantly longer in G1-2 than G3 tumors with the logrank test (P <0.03) and multivariate analysis. Conclusions: Our results seem to stress the difficulty to individualize different prognostic subsets from a series of breast cancer patients with only visceral metastases at initial diagnosis according to DNA flow cytometry and nuclear grade.

AB - Aims and background: The presence of early metastases to distant sites in breast cancer patients is an infrequent event whose mechanisms are still not clear. The aim of this study was to evaluate the biologic and clinical role of DNA ploidy and cell nuclear grade of primary tumors in the metastatic process of a series of stage IV previously untreated breast cancer patients with only visceral metastases. Methods: DNA flow cytometry analysis on paraffin-embedded material and cell nuclear grading of primary tumors was performed on a series of 50 breast cancer patients with only visceral metastases at the time of initial diagnosis. Results: Aneuploidy was found in 28/46 (61%) of evaluable cases and was independent of site of involvement, clinical response, time to progression and overall survival of patients. Of the 46 cases evaluable for nuclear grade, 5 (11%), 16 (35%) and 25 (54%) were classified as G1 (well-differentiated) G2 and G3, respectively. Nuclear grade also was unrelated to response to therapy and overall survival, whereas time to progression was significantly longer in G1-2 than G3 tumors with the logrank test (P <0.03) and multivariate analysis. Conclusions: Our results seem to stress the difficulty to individualize different prognostic subsets from a series of breast cancer patients with only visceral metastases at initial diagnosis according to DNA flow cytometry and nuclear grade.

KW - breast cancer

KW - nuclear grade

KW - ploidy

KW - visceral metastases

UR - http://www.scopus.com/inward/record.url?scp=10244267480&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10244267480&partnerID=8YFLogxK

M3 - Article

VL - 82

SP - 386

EP - 389

JO - Tumori

JF - Tumori

SN - 0300-8916

IS - 4

ER -