Nuclear localization of cancer stem cell marker CD133 in triple-negative breast cancer

A case report

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aim and background. It has been recently demonstrated that the detection of stem cell niches in triple-negative (TN) breast cancer may provide good prognostic clues for this tumor. Methods and study design.We investigated the subcellular expression and localization of the cancer stem cell marker CD133 in a TN breast cancer biopsy from a 42- year-old Caucasian woman with a histological diagnosis of high-grade invasive ductal breast carcinoma by immunohistochemistry, flow cytometry and quantitative real- time PCR (qRT-PCR). Results.We describe for the first time in a TN breast cancer the nuclear mislocalization of CD133, which normally shows membrane localization and more sporadically cytoplasmic localization. We also found this aberrant expression with qRT-PCR analysis but not flow cytometry. Conclusions. Nuclear localization of CD133 may be an indicator of poor prognosis in TN breast cancer, as it is known that surface molecules, when moving into the nucleus, can act as transcriptional regulators by interfering with molecular pathways directly connected to the proliferation and differentiation of tumor cells.

Original languageEnglish
JournalTumori
Volume99
Issue number5
DOIs
Publication statusPublished - Sep 2013

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Triple Negative Breast Neoplasms
Neoplastic Stem Cells
Real-Time Polymerase Chain Reaction
Flow Cytometry
Stem Cell Niche
Carcinoma, Ductal, Breast
Cell Differentiation
Neoplasms
Immunohistochemistry
Biopsy
Membranes

Keywords

  • CD133
  • Nuclear localization
  • Triple-negative breast cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

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title = "Nuclear localization of cancer stem cell marker CD133 in triple-negative breast cancer: A case report",
abstract = "Aim and background. It has been recently demonstrated that the detection of stem cell niches in triple-negative (TN) breast cancer may provide good prognostic clues for this tumor. Methods and study design.We investigated the subcellular expression and localization of the cancer stem cell marker CD133 in a TN breast cancer biopsy from a 42- year-old Caucasian woman with a histological diagnosis of high-grade invasive ductal breast carcinoma by immunohistochemistry, flow cytometry and quantitative real- time PCR (qRT-PCR). Results.We describe for the first time in a TN breast cancer the nuclear mislocalization of CD133, which normally shows membrane localization and more sporadically cytoplasmic localization. We also found this aberrant expression with qRT-PCR analysis but not flow cytometry. Conclusions. Nuclear localization of CD133 may be an indicator of poor prognosis in TN breast cancer, as it is known that surface molecules, when moving into the nucleus, can act as transcriptional regulators by interfering with molecular pathways directly connected to the proliferation and differentiation of tumor cells.",
keywords = "CD133, Nuclear localization, Triple-negative breast cancer",
author = "Monica Cantile and Francesca Collina and Massimiliano D'Aiuto and Massimo Rinaldo and Giuseppe Pirozzi and Carmela Borsellino and Renato Franco and Gerardo Botti and {Di Bonito}, Maurizio",
year = "2013",
month = "9",
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language = "English",
volume = "99",
journal = "Tumori",
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TY - JOUR

T1 - Nuclear localization of cancer stem cell marker CD133 in triple-negative breast cancer

T2 - A case report

AU - Cantile, Monica

AU - Collina, Francesca

AU - D'Aiuto, Massimiliano

AU - Rinaldo, Massimo

AU - Pirozzi, Giuseppe

AU - Borsellino, Carmela

AU - Franco, Renato

AU - Botti, Gerardo

AU - Di Bonito, Maurizio

PY - 2013/9

Y1 - 2013/9

N2 - Aim and background. It has been recently demonstrated that the detection of stem cell niches in triple-negative (TN) breast cancer may provide good prognostic clues for this tumor. Methods and study design.We investigated the subcellular expression and localization of the cancer stem cell marker CD133 in a TN breast cancer biopsy from a 42- year-old Caucasian woman with a histological diagnosis of high-grade invasive ductal breast carcinoma by immunohistochemistry, flow cytometry and quantitative real- time PCR (qRT-PCR). Results.We describe for the first time in a TN breast cancer the nuclear mislocalization of CD133, which normally shows membrane localization and more sporadically cytoplasmic localization. We also found this aberrant expression with qRT-PCR analysis but not flow cytometry. Conclusions. Nuclear localization of CD133 may be an indicator of poor prognosis in TN breast cancer, as it is known that surface molecules, when moving into the nucleus, can act as transcriptional regulators by interfering with molecular pathways directly connected to the proliferation and differentiation of tumor cells.

AB - Aim and background. It has been recently demonstrated that the detection of stem cell niches in triple-negative (TN) breast cancer may provide good prognostic clues for this tumor. Methods and study design.We investigated the subcellular expression and localization of the cancer stem cell marker CD133 in a TN breast cancer biopsy from a 42- year-old Caucasian woman with a histological diagnosis of high-grade invasive ductal breast carcinoma by immunohistochemistry, flow cytometry and quantitative real- time PCR (qRT-PCR). Results.We describe for the first time in a TN breast cancer the nuclear mislocalization of CD133, which normally shows membrane localization and more sporadically cytoplasmic localization. We also found this aberrant expression with qRT-PCR analysis but not flow cytometry. Conclusions. Nuclear localization of CD133 may be an indicator of poor prognosis in TN breast cancer, as it is known that surface molecules, when moving into the nucleus, can act as transcriptional regulators by interfering with molecular pathways directly connected to the proliferation and differentiation of tumor cells.

KW - CD133

KW - Nuclear localization

KW - Triple-negative breast cancer

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