Nuclear localization of phosphorylated c-Myc protein in human tumor cells

C. Soldani, M. G. Bottone, M. Biggiogera, C. Alpini, A. I. Scovassi, T. Martin, C. Pellicciari

Research output: Contribution to journalArticle

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Abstract

Using immunocytochemical techniques at light and electron microscopy, we analysed the distribution of phosphorylated c-Myc in actively proliferating human HeLa cells. The distribution pattern of c-Myc was also compared with those of other ribonucleoprotein (RNP)-containing components (PANA, hnRNP-core proteins, fibrillarin) or RNP-associated nuclear proteins (SC-35 splicing factor). Our results provide the first evidence that phosphorylated c-Myc accumulates in the nucleus of tumor cells, where it colocalizes with fibrillarin, both in the nucleolus and in extranucleolar structures.

Original languageEnglish
Pages (from-to)377-380
Number of pages4
JournalEuropean journal of histochemistry : EJH
Volume46
Issue number4
Publication statusPublished - 2002

Fingerprint

ribonucleoproteins
Ribonucleoproteins
Heterogeneous-Nuclear Ribonucleoproteins
nuclear proteins
cell nucleolus
Nuclear Proteins
Cell Nucleus
HeLa Cells
light microscopy
Neoplasms
electron microscopy
Electron Microscopy
proteins
Light
cells
human MYC protein
fibrillarin
neoplasm cells
methodology
RNA Splicing Factors

Keywords

  • C-Myc
  • Fibrillarin
  • Fluorescence microscopy
  • Immunocytochemistry
  • Nuclear bodies
  • Nucleolus

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Animal Science and Zoology
  • Developmental Biology

Cite this

Soldani, C., Bottone, M. G., Biggiogera, M., Alpini, C., Scovassi, A. I., Martin, T., & Pellicciari, C. (2002). Nuclear localization of phosphorylated c-Myc protein in human tumor cells. European journal of histochemistry : EJH, 46(4), 377-380.

Nuclear localization of phosphorylated c-Myc protein in human tumor cells. / Soldani, C.; Bottone, M. G.; Biggiogera, M.; Alpini, C.; Scovassi, A. I.; Martin, T.; Pellicciari, C.

In: European journal of histochemistry : EJH, Vol. 46, No. 4, 2002, p. 377-380.

Research output: Contribution to journalArticle

Soldani, C, Bottone, MG, Biggiogera, M, Alpini, C, Scovassi, AI, Martin, T & Pellicciari, C 2002, 'Nuclear localization of phosphorylated c-Myc protein in human tumor cells', European journal of histochemistry : EJH, vol. 46, no. 4, pp. 377-380.
Soldani, C. ; Bottone, M. G. ; Biggiogera, M. ; Alpini, C. ; Scovassi, A. I. ; Martin, T. ; Pellicciari, C. / Nuclear localization of phosphorylated c-Myc protein in human tumor cells. In: European journal of histochemistry : EJH. 2002 ; Vol. 46, No. 4. pp. 377-380.
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