Nuclear metabolism. I. Determination of styrene monooxygenase activity in rat liver nuclei

G. Gazzotti, E. Garattini, M. Salmona

Research output: Contribution to journalArticle

Abstract

Styrene monooxygenase activity was measured in intact nuclear preparations from rat liver by means of a gas chromatographic method. Styrene epoxide formation is NADPH-dependent although it is enhanced when NADH is added with NADPH. This activity is inhibited by microsomal monooxygenase inhibitors SKF 525A and metyrapone and by microsomal epoxide hydrase inhibitors 1,2-epoxy-3,3,3-trichloropropene oxide and cyclohexene oxide. The percentage of inhibition is quantitatively dffferent for the four compounds. Known inducers of liver microsomal monooxygenase show different patterns of induction on nuclear preparations. Phenobarbital induces nuclear monooxygenase activity more than the respective microsomal activity, whereas the contrary holds true for β-naphthoflavone.

Original languageEnglish
Pages (from-to)189-195
Number of pages7
JournalChemico-Biological Interactions
Volume29
Issue number2
DOIs
Publication statusPublished - 1980

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'Nuclear metabolism. I. Determination of styrene monooxygenase activity in rat liver nuclei'. Together they form a unique fingerprint.

  • Cite this