Nuclear phosphoinositide-specific phospholipase C, phosphatidylinositol 4,5-bisphosphate and protein kinase C during rat spermatogenesis

Elisabetta Caramelli, Alessandro Matteucci, Nicoletta Zini, Cinzia Carini, Lia Guidotti, Daniele Ricci, Nadir M. Maraldi, Silvano Capitani

Research output: Contribution to journalArticle

Abstract

Presence and intracellular distribution of phosphoinositide-specific phospholipase C, phosphatidylinositol 4,5-bisphosphate and protein kinase C have been investigated in rat maturing germ cells and spermatozoa. The isoforms β1 and γ1 of phosphoinositide-specific phospholipase C were immunologically identified and found to be predominantly nuclear or cytoplasmic and nuclear, respectively. The two enzymes mere present in the maturing cell lineage of the seminiferous tubule, except for the nucleus of late spermatids, and absent in spermatozoa, in which, however, a phosphoinositide-specific phospholipase C activity persisted, due to yet uncharacterized enzyme(s). Protein kinase C paralleled these developmental changes, and was completely down-regulated in both total cell homogenates and isolated nuclei obtained from spermatozoa. On the contrary, phosphatidylinositol 4,5-bisphosphate, present at the nuclear level in all cell types, accumulated in the nuclei of late spermatids and spermatozoa. These data support the contention that the spermatozoon nucleus stores a lipid-dependent signaling apparatus which could be reactivated either during sperm maturation or at fertilization.

Original languageEnglish
Pages (from-to)154-164
Number of pages11
JournalEuropean Journal of Cell Biology
Volume71
Issue number2
Publication statusPublished - 1996

Keywords

  • Nucleus
  • Phospholipase C
  • PKC differentiation
  • Rat
  • Spermatozoa

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

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  • Cite this

    Caramelli, E., Matteucci, A., Zini, N., Carini, C., Guidotti, L., Ricci, D., Maraldi, N. M., & Capitani, S. (1996). Nuclear phosphoinositide-specific phospholipase C, phosphatidylinositol 4,5-bisphosphate and protein kinase C during rat spermatogenesis. European Journal of Cell Biology, 71(2), 154-164.