TY - JOUR
T1 - Nuclear PLCβ1 acts as a negative regulator of p45/NF-E2 expression levels in Friend erythroleukemia cells
AU - Faenza, Irene
AU - Matteucci, Alessandro
AU - Bavelloni, Alberto
AU - Marmiroli, Sandra
AU - Martelli, Alberto M.
AU - Gilmour, R. Stewart
AU - Suh, Pann Ghill
AU - Manzoli, Lucia
AU - Cocco, Lucio
PY - 2002/5/8
Y1 - 2002/5/8
N2 - It is well established that phospholipase C (PLC) β1 plays a role in the nuclear compartment and is involved in the signalling pathway that controls the switching of the erythroleukemia cells programming from an undifferentiated to a differentiated state. Constitutive overexpression of nuclear PLCβ1 has been previously shown to inhibit Friend cells differentiation. For further characterization, we investigated the localization of PLCβ1a and PLCβ1b in Friend cells by fusing their cDNA to enhanced green fluorescent protein (GFP). To investigate the potential target of nuclear PLCβ1 in Friend differentiation, we studied the expression of p45/NF-E2 transcription factor, which is an enhancer binding protein for expression of the β-globin gene and the expression of GATA proteins that are important for the survival and differentiation of erythroid cells. Our data suggest that the overexpression of PLCβ1 (both 1a and 1b) only in the nuclear compartment significantly reduces the expression of p45/NF-E2. The effect observed is attributable to the specific action of nuclear PLCβ1 signalling given that GATA-1 and GATA-3 are not affected at all. Here we show the existence of a unique target, i.e. the transcription factor p45/NF-E2, whose expression is specifically inhibited by the nuclear signalling evoked by PLCβ1 forms.
AB - It is well established that phospholipase C (PLC) β1 plays a role in the nuclear compartment and is involved in the signalling pathway that controls the switching of the erythroleukemia cells programming from an undifferentiated to a differentiated state. Constitutive overexpression of nuclear PLCβ1 has been previously shown to inhibit Friend cells differentiation. For further characterization, we investigated the localization of PLCβ1a and PLCβ1b in Friend cells by fusing their cDNA to enhanced green fluorescent protein (GFP). To investigate the potential target of nuclear PLCβ1 in Friend differentiation, we studied the expression of p45/NF-E2 transcription factor, which is an enhancer binding protein for expression of the β-globin gene and the expression of GATA proteins that are important for the survival and differentiation of erythroid cells. Our data suggest that the overexpression of PLCβ1 (both 1a and 1b) only in the nuclear compartment significantly reduces the expression of p45/NF-E2. The effect observed is attributable to the specific action of nuclear PLCβ1 signalling given that GATA-1 and GATA-3 are not affected at all. Here we show the existence of a unique target, i.e. the transcription factor p45/NF-E2, whose expression is specifically inhibited by the nuclear signalling evoked by PLCβ1 forms.
KW - Erythroleukemia
KW - GATA family
KW - Inositol lipid
KW - NF-E2
KW - Nucleus
KW - Phospholipase Cβ
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UR - http://www.scopus.com/inward/citedby.url?scp=0037042379&partnerID=8YFLogxK
U2 - 10.1016/S0167-4889(02)00192-1
DO - 10.1016/S0167-4889(02)00192-1
M3 - Article
C2 - 12031797
AN - SCOPUS:0037042379
VL - 1589
SP - 305
EP - 310
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
SN - 0167-4889
IS - 3
ER -