Nuclear translocation of insulin receptor substrate-1 by oncogenes and Igf-I

Effect on ribosomal RNA synthesis

Xiao Tu, Priti Batta, Nathalie Innocent, Marco Prisco, Ivan Casaburi, Barbara Belletti, Renato Baserga

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

The insulin receptor substrate-1 (IRS-1) is one of the major substrates of both the insulin and IGF-I receptors and is generally localized in the cytosol/membrane fraction of the cell. We show here that a substantial fraction of IRS-1 is translocated to the nucleus in mouse embryo fibroblasts (MEF) expressing the simian virus 40 T antigen. Nuclear translocation of IRS-1 occurs also in MEF stimulated with IGF-I or in MEF expressing the oncogene v-src. Nuclear translocation of IRS-1 can be demonstrated by confocal microscopy, immunohistochemistry, or subcellular fractionation. An antibody to IRS-1 immunoprecipitates from nuclear fractions (but not from cytosolic fractions) the upstream binding factor, which is a key regulator of RNA polymerase I activity and ribosomal RNA (rRNA) synthesis. In agreement with this finding, in 32D murine hemopoietic cells, nuclear translocation of IRS-1 correlates with a markedly increased rRNA synthesis. Our experiments suggest that nuclear IRS-1 may play a specialized role in rRNA synthesis and/or processing.

Original languageEnglish
Pages (from-to)44357-44365
Number of pages9
JournalJournal of Biological Chemistry
Volume277
Issue number46
DOIs
Publication statusPublished - Nov 15 2002

Fingerprint

Insulin Receptor Substrate Proteins
Oncogenes
Fibroblasts
Ribosomal RNA
Embryonic Structures
src Genes
IGF Type 1 Receptor
Simian virus 40
Confocal microscopy
Viral Tumor Antigens
Cytoplasmic and Nuclear Receptors
Fractionation
Insulin-Like Growth Factor I
Viruses
Confocal Microscopy
Cytosol
Immunohistochemistry
Cell Membrane
Insulin
Membranes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Nuclear translocation of insulin receptor substrate-1 by oncogenes and Igf-I : Effect on ribosomal RNA synthesis. / Tu, Xiao; Batta, Priti; Innocent, Nathalie; Prisco, Marco; Casaburi, Ivan; Belletti, Barbara; Baserga, Renato.

In: Journal of Biological Chemistry, Vol. 277, No. 46, 15.11.2002, p. 44357-44365.

Research output: Contribution to journalArticle

Tu, Xiao ; Batta, Priti ; Innocent, Nathalie ; Prisco, Marco ; Casaburi, Ivan ; Belletti, Barbara ; Baserga, Renato. / Nuclear translocation of insulin receptor substrate-1 by oncogenes and Igf-I : Effect on ribosomal RNA synthesis. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 46. pp. 44357-44365.
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