Nucleocytoplasmic shuttling of endocytic proteins

Manuela Vecchi, Simona Polo, Viviane Poupon, Jan Willem Van De Loo, Alexandre Benmerah, Pier Paolo Di Fiore

Research output: Contribution to journalArticle

Abstract

Many cellular processes rely on the ordered assembly of macromolecular structures. Here, we uncover an unexpected link between two such processes, endocytosis and transcription. Many endocytic proteins, including eps15, epsin1, the clathrin assembly lymphoid myeloid leukemia (CALM), and α-adaptin, accumulate in the nucleus when nuclear export is inhibited. Endocytosis and nucleocytoplasmic shuttling of endocytic proteins are apparently independent processes, since inhibition of endocytosis did not appreciably alter nuclear translocation of endocytic proteins, and blockade of nuclear export did not change the initial rate of endocytosis. In the nucleus, eps15 and CALM acted as positive modulators of transcription in a GAL4-based transactivation assay, thus raising the intriguing possibility that some endocytic proteins play a direct or indirect role in transcriptional regulation.

Original languageEnglish
Pages (from-to)1511-1517
Number of pages7
JournalJournal of Cell Biology
Volume153
Issue number7
DOIs
Publication statusPublished - Jun 26 2001

Keywords

  • CRM1
  • Endocytosis
  • eps15
  • Nucleocytoplasmic shuttling
  • Transcription

ASJC Scopus subject areas

  • Cell Biology

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