Abstract
Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK response element (JRE) in the 5' untranslated region (UTR). We have now identified two major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and for JNK-induced stabilization in a cell-free system that duplicates essential features of regulated mRNA decay. Nucleolin and YB. 1 are required for formation of an IL-2 mRNP complex that responds to specific mRNA stabilizing signals.
| Original language | English |
|---|---|
| Pages (from-to) | 1236-1248 |
| Number of pages | 13 |
| Journal | Genes and Development |
| Volume | 14 |
| Issue number | 10 |
| Publication status | Published - May 15 2000 |
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Keywords
- JNK
- Nucleolin
- Stabilization
- T-cell activation
- Trans-acting factors
ASJC Scopus subject areas
- Genetics
- Developmental Biology
Cite this
Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation. / Chen, Ching Yi; Gherzi, Roberto; Andersen, Jens S.; Gaietta, Guido; Jürchott, Karsten; Royer, Hans Dieter; Mann, Matthias; Karin, Michael.
In: Genes and Development, Vol. 14, No. 10, 15.05.2000, p. 1236-1248.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation
AU - Chen, Ching Yi
AU - Gherzi, Roberto
AU - Andersen, Jens S.
AU - Gaietta, Guido
AU - Jürchott, Karsten
AU - Royer, Hans Dieter
AU - Mann, Matthias
AU - Karin, Michael
PY - 2000/5/15
Y1 - 2000/5/15
N2 - Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK response element (JRE) in the 5' untranslated region (UTR). We have now identified two major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and for JNK-induced stabilization in a cell-free system that duplicates essential features of regulated mRNA decay. Nucleolin and YB. 1 are required for formation of an IL-2 mRNP complex that responds to specific mRNA stabilizing signals.
AB - Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK response element (JRE) in the 5' untranslated region (UTR). We have now identified two major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and for JNK-induced stabilization in a cell-free system that duplicates essential features of regulated mRNA decay. Nucleolin and YB. 1 are required for formation of an IL-2 mRNP complex that responds to specific mRNA stabilizing signals.
KW - JNK
KW - Nucleolin
KW - Stabilization
KW - T-cell activation
KW - Trans-acting factors
UR - http://www.scopus.com/inward/record.url?scp=0034657357&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034657357&partnerID=8YFLogxK
M3 - Article
C2 - 10817758
AN - SCOPUS:0034657357
VL - 14
SP - 1236
EP - 1248
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 10
ER -