Abstract
Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK response element (JRE) in the 5' untranslated region (UTR). We have now identified two major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and for JNK-induced stabilization in a cell-free system that duplicates essential features of regulated mRNA decay. Nucleolin and YB. 1 are required for formation of an IL-2 mRNP complex that responds to specific mRNA stabilizing signals.
Original language | English |
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Pages (from-to) | 1236-1248 |
Number of pages | 13 |
Journal | Genes and Development |
Volume | 14 |
Issue number | 10 |
Publication status | Published - May 15 2000 |
Keywords
- JNK
- Nucleolin
- Stabilization
- T-cell activation
- Trans-acting factors
ASJC Scopus subject areas
- Genetics
- Developmental Biology