Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation

Ching Yi Chen, Roberto Gherzi, Jens S. Andersen, Guido Gaietta, Karsten Jürchott, Hans Dieter Royer, Matthias Mann, Michael Karin

Research output: Contribution to journalArticlepeer-review

Abstract

Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK response element (JRE) in the 5' untranslated region (UTR). We have now identified two major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and for JNK-induced stabilization in a cell-free system that duplicates essential features of regulated mRNA decay. Nucleolin and YB. 1 are required for formation of an IL-2 mRNP complex that responds to specific mRNA stabilizing signals.

Original languageEnglish
Pages (from-to)1236-1248
Number of pages13
JournalGenes and Development
Volume14
Issue number10
Publication statusPublished - May 15 2000

Keywords

  • JNK
  • Nucleolin
  • Stabilization
  • T-cell activation
  • Trans-acting factors

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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