Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation

Ching Yi Chen; Roberto Gherzi; Jens S. Andersen; Guido Gaietta; Karsten Jürchott; Hans Dieter Royer; Matthias Mann; Michael Karin

Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation

Ching Yi Chen, Roberto Gherzi, Jens S. Andersen, Guido Gaietta, Karsten Jürchott, Hans Dieter Royer, Matthias Mann, Michael Karin

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article › peer-review

Abstract

Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK response element (JRE) in the 5' untranslated region (UTR). We have now identified two major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and for JNK-induced stabilization in a cell-free system that duplicates essential features of regulated mRNA decay. Nucleolin and YB. 1 are required for formation of an IL-2 mRNP complex that responds to specific mRNA stabilizing signals.

Original language	English
Pages (from-to)	1236-1248
Number of pages	13
Journal	Genes and Development
Volume	14
Issue number	10
Publication status	Published - May 15 2000

Keywords

JNK
Nucleolin
Stabilization
T-cell activation
Trans-acting factors

ASJC Scopus subject areas

Genetics
Developmental Biology

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Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation. / Chen, Ching Yi; Gherzi, Roberto; Andersen, Jens S.; Gaietta, Guido; Jürchott, Karsten; Royer, Hans Dieter; Mann, Matthias; Karin, Michael.

In: Genes and Development, Vol. 14, No. 10, 15.05.2000, p. 1236-1248.

Research output: Contribution to journal › Article › peer-review

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abstract = "Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK response element (JRE) in the 5' untranslated region (UTR). We have now identified two major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and for JNK-induced stabilization in a cell-free system that duplicates essential features of regulated mRNA decay. Nucleolin and YB. 1 are required for formation of an IL-2 mRNP complex that responds to specific mRNA stabilizing signals.",

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AU - Gaietta, Guido

AU - Jürchott, Karsten

AU - Royer, Hans Dieter

AU - Mann, Matthias

AU - Karin, Michael

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AB - Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK response element (JRE) in the 5' untranslated region (UTR). We have now identified two major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and for JNK-induced stabilization in a cell-free system that duplicates essential features of regulated mRNA decay. Nucleolin and YB. 1 are required for formation of an IL-2 mRNP complex that responds to specific mRNA stabilizing signals.

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Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation

Abstract

Keywords

ASJC Scopus subject areas

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