Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation

Ching Yi Chen, Roberto Gherzi, Jens S. Andersen, Guido Gaietta, Karsten Jürchott, Hans Dieter Royer, Matthias Mann, Michael Karin

Research output: Contribution to journalArticle

290 Citations (Scopus)

Abstract

Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK response element (JRE) in the 5' untranslated region (UTR). We have now identified two major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and for JNK-induced stabilization in a cell-free system that duplicates essential features of regulated mRNA decay. Nucleolin and YB. 1 are required for formation of an IL-2 mRNP complex that responds to specific mRNA stabilizing signals.

Original languageEnglish
Pages (from-to)1236-1248
Number of pages13
JournalGenes and Development
Volume14
Issue number10
Publication statusPublished - May 15 2000

Fingerprint

Interleukin-2
T-Lymphocytes
Messenger RNA
Response Elements
RNA-Binding Proteins
Cell-Free System
MAP Kinase Signaling System
5' Untranslated Regions
RNA Stability
Carrier Proteins
nucleolin

Keywords

  • JNK
  • Nucleolin
  • Stabilization
  • T-cell activation
  • Trans-acting factors

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Chen, C. Y., Gherzi, R., Andersen, J. S., Gaietta, G., Jürchott, K., Royer, H. D., ... Karin, M. (2000). Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation. Genes and Development, 14(10), 1236-1248.

Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation. / Chen, Ching Yi; Gherzi, Roberto; Andersen, Jens S.; Gaietta, Guido; Jürchott, Karsten; Royer, Hans Dieter; Mann, Matthias; Karin, Michael.

In: Genes and Development, Vol. 14, No. 10, 15.05.2000, p. 1236-1248.

Research output: Contribution to journalArticle

Chen, CY, Gherzi, R, Andersen, JS, Gaietta, G, Jürchott, K, Royer, HD, Mann, M & Karin, M 2000, 'Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation', Genes and Development, vol. 14, no. 10, pp. 1236-1248.
Chen CY, Gherzi R, Andersen JS, Gaietta G, Jürchott K, Royer HD et al. Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation. Genes and Development. 2000 May 15;14(10):1236-1248.
Chen, Ching Yi ; Gherzi, Roberto ; Andersen, Jens S. ; Gaietta, Guido ; Jürchott, Karsten ; Royer, Hans Dieter ; Mann, Matthias ; Karin, Michael. / Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation. In: Genes and Development. 2000 ; Vol. 14, No. 10. pp. 1236-1248.
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