Abstract
Nucleophosmin (NPM) is a ubiquitously expressed nucleolar phosphoprotein that continuously shuttles between the nucleus and cytoplasm1. It has been proposed to function in ribosomal protein assembly and transport2, and also as a molecular chaperone that prevents proteins from aggregating in the crowded environment of the nucleolus3. The NPM gene is involved in several tumour-associated chromosome translocations, which have resulted in the formation of fusion proteins that retain the amino terminus of NPM, including NPM-ALK4, NPM-RAR5 and NPM-MLF1 (ref. 6). It is generally thought that the NPM component is not involved in the transforming potential of these fusion proteins7, but instead provides a dimerization interface for the oligomerization and the oncogenic conversion of the various NPM partners (ALK, RAR, MLF1). Here we show that NPM interacts directly with the tumour suppressor p53, regulates the increase in stability and transcriptional activation of p53 after different types of stress, and induces p53-dependent premature senescence on overexpression in diploid fibroblasts. These findings indicate that NPM is a crucial regulator of p53 and suggest that alterations of the NPM function by NPM fusion proteins might lead to deregulation of p53 in tumours.
| Original language | English |
|---|---|
| Pages (from-to) | 529-533 |
| Number of pages | 5 |
| Journal | Nature Cell Biology |
| Volume | 4 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2002 |
Fingerprint
ASJC Scopus subject areas
- Cell Biology
Cite this
Nucleophosmin regulates the stability and transcriptional activity of p53. / Colombo, Emanuela; Marine, Jean Christophe; Danovi, Davide; Falini, Brunangelo; Pelicci, Pier Giuseppe.
In: Nature Cell Biology, Vol. 4, No. 7, 2002, p. 529-533.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nucleophosmin regulates the stability and transcriptional activity of p53
AU - Colombo, Emanuela
AU - Marine, Jean Christophe
AU - Danovi, Davide
AU - Falini, Brunangelo
AU - Pelicci, Pier Giuseppe
PY - 2002
Y1 - 2002
N2 - Nucleophosmin (NPM) is a ubiquitously expressed nucleolar phosphoprotein that continuously shuttles between the nucleus and cytoplasm1. It has been proposed to function in ribosomal protein assembly and transport2, and also as a molecular chaperone that prevents proteins from aggregating in the crowded environment of the nucleolus3. The NPM gene is involved in several tumour-associated chromosome translocations, which have resulted in the formation of fusion proteins that retain the amino terminus of NPM, including NPM-ALK4, NPM-RAR5 and NPM-MLF1 (ref. 6). It is generally thought that the NPM component is not involved in the transforming potential of these fusion proteins7, but instead provides a dimerization interface for the oligomerization and the oncogenic conversion of the various NPM partners (ALK, RAR, MLF1). Here we show that NPM interacts directly with the tumour suppressor p53, regulates the increase in stability and transcriptional activation of p53 after different types of stress, and induces p53-dependent premature senescence on overexpression in diploid fibroblasts. These findings indicate that NPM is a crucial regulator of p53 and suggest that alterations of the NPM function by NPM fusion proteins might lead to deregulation of p53 in tumours.
AB - Nucleophosmin (NPM) is a ubiquitously expressed nucleolar phosphoprotein that continuously shuttles between the nucleus and cytoplasm1. It has been proposed to function in ribosomal protein assembly and transport2, and also as a molecular chaperone that prevents proteins from aggregating in the crowded environment of the nucleolus3. The NPM gene is involved in several tumour-associated chromosome translocations, which have resulted in the formation of fusion proteins that retain the amino terminus of NPM, including NPM-ALK4, NPM-RAR5 and NPM-MLF1 (ref. 6). It is generally thought that the NPM component is not involved in the transforming potential of these fusion proteins7, but instead provides a dimerization interface for the oligomerization and the oncogenic conversion of the various NPM partners (ALK, RAR, MLF1). Here we show that NPM interacts directly with the tumour suppressor p53, regulates the increase in stability and transcriptional activation of p53 after different types of stress, and induces p53-dependent premature senescence on overexpression in diploid fibroblasts. These findings indicate that NPM is a crucial regulator of p53 and suggest that alterations of the NPM function by NPM fusion proteins might lead to deregulation of p53 in tumours.
UR - http://www.scopus.com/inward/record.url?scp=0036302062&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036302062&partnerID=8YFLogxK
U2 - 10.1038/ncb814
DO - 10.1038/ncb814
M3 - Article
C2 - 12080348
AN - SCOPUS:0036302062
VL - 4
SP - 529
EP - 533
JO - Nature Cell Biology
JF - Nature Cell Biology
SN - 1465-7392
IS - 7
ER -