TY - JOUR
T1 - Nucleoplasmic Lamin A/C and Polycomb group of proteins
T2 - an evolutionarily conserved interplay
AU - Marullo, F.
AU - Cesarini, E.
AU - Antonelli, L.
AU - Gregoretti, F.
AU - Oliva, G.
AU - Lanzuolo, C.
PY - 2016/4/28
Y1 - 2016/4/28
N2 - Nuclear lamins are the main components of the nuclear lamina at the nuclear periphery, providing mechanical support to the nucleus. However, recent findings suggest that lamins also reside in the nuclear interior, as a distinct and dynamic pool with critical roles in transcriptional regulation. In our work we found a functional and evolutionary conserved crosstalk between Lamin A/C and the Polycomb group (PcG) of proteins, this being required for the maintenance of the PcG repressive functions. Indeed, Lamin A/C knock-down causes PcG foci dispersion and defects in PcG-mediated higher order structures, thereby leading to impaired PcG mediated transcriptional repression. By using ad-hoc algorithms for image analysis and PLA approaches we hereby show that PcG proteins are preferentially located in the nuclear interior where they interact with nucleoplasmic Lamin A/C. Taken together, our findings suggest that nuclear components, such as Lamin A/C, functionally interact with epigenetic factors to ensure the correct transcriptional program maintenance.
AB - Nuclear lamins are the main components of the nuclear lamina at the nuclear periphery, providing mechanical support to the nucleus. However, recent findings suggest that lamins also reside in the nuclear interior, as a distinct and dynamic pool with critical roles in transcriptional regulation. In our work we found a functional and evolutionary conserved crosstalk between Lamin A/C and the Polycomb group (PcG) of proteins, this being required for the maintenance of the PcG repressive functions. Indeed, Lamin A/C knock-down causes PcG foci dispersion and defects in PcG-mediated higher order structures, thereby leading to impaired PcG mediated transcriptional repression. By using ad-hoc algorithms for image analysis and PLA approaches we hereby show that PcG proteins are preferentially located in the nuclear interior where they interact with nucleoplasmic Lamin A/C. Taken together, our findings suggest that nuclear components, such as Lamin A/C, functionally interact with epigenetic factors to ensure the correct transcriptional program maintenance.
KW - Lamin A/C
KW - nuclear architecture
KW - nucleoplasm
KW - Polycomb
KW - transcription
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U2 - 10.1080/19491034.2016.1157675
DO - 10.1080/19491034.2016.1157675
M3 - Article
AN - SCOPUS:84964663768
SP - 1
EP - 9
JO - Nucleus
JF - Nucleus
SN - 0115-2300
ER -