Nucleosomal domains of mouse spermatozoa chromatin as potential sites for retroposition and foreign DNA integration

Carmine Pittoggi, Germana Zaccagnini, Roberto Giordano, Anna Rosa Magnano, Baccio Baccetti, Rodolfo Lorenzini, Corrado Spadafora

Research output: Contribution to journalArticle

Abstract

Exogenous DNA molecules are spontaneously taken up by sperm cells, internalized in nuclei, and eventually integrated in the sperm genome. The actual occurrence of the integration suggests that the sperm chromosomal DNA is not uniformly and tightly packed with protamines, implying the existence of genomic sites where the chromosomal DNA is accessible to foreign molecules. We have characterized a hypersensitive, nucleosomal subfraction of mouse sperm chromatin that is highly enriched in unmethylated retroposon DNA from a variety of families. Here we propose that both the integration of exogenous DNA molecules, and the endogenous retroposition activity, occur in the same site(s) of sperm chromatin. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)248-251
Number of pages4
JournalMolecular Reproduction and Development
Volume56
Issue number2 SUPPL.
Publication statusPublished - 2000

Keywords

  • Nuclease hypersensitivity
  • Nucleosomes
  • Retroposon DNA
  • Sperm cell chromatin

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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  • Cite this

    Pittoggi, C., Zaccagnini, G., Giordano, R., Magnano, A. R., Baccetti, B., Lorenzini, R., & Spadafora, C. (2000). Nucleosomal domains of mouse spermatozoa chromatin as potential sites for retroposition and foreign DNA integration. Molecular Reproduction and Development, 56(2 SUPPL.), 248-251.