Abstract
Exogenous DNA molecules are spontaneously taken up by sperm cells, internalized in nuclei, and eventually integrated in the sperm genome. The actual occurrence of the integration suggests that the sperm chromosomal DNA is not uniformly and tightly packed with protamines, implying the existence of genomic sites where the chromosomal DNA is accessible to foreign molecules. We have characterized a hypersensitive, nucleosomal subfraction of mouse sperm chromatin that is highly enriched in unmethylated retroposon DNA from a variety of families. Here we propose that both the integration of exogenous DNA molecules, and the endogenous retroposition activity, occur in the same site(s) of sperm chromatin. (C) 2000 Wiley-Liss, Inc.
| Original language | English |
|---|---|
| Pages (from-to) | 248-251 |
| Number of pages | 4 |
| Journal | Molecular Reproduction and Development |
| Volume | 56 |
| Issue number | 2 SUPPL. |
| Publication status | Published - 2000 |
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Keywords
- Nuclease hypersensitivity
- Nucleosomes
- Retroposon DNA
- Sperm cell chromatin
ASJC Scopus subject areas
- Genetics
- Developmental Biology
- Cell Biology
Cite this
Nucleosomal domains of mouse spermatozoa chromatin as potential sites for retroposition and foreign DNA integration. / Pittoggi, Carmine; Zaccagnini, Germana; Giordano, Roberto; Magnano, Anna Rosa; Baccetti, Baccio; Lorenzini, Rodolfo; Spadafora, Corrado.
In: Molecular Reproduction and Development, Vol. 56, No. 2 SUPPL., 2000, p. 248-251.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nucleosomal domains of mouse spermatozoa chromatin as potential sites for retroposition and foreign DNA integration
AU - Pittoggi, Carmine
AU - Zaccagnini, Germana
AU - Giordano, Roberto
AU - Magnano, Anna Rosa
AU - Baccetti, Baccio
AU - Lorenzini, Rodolfo
AU - Spadafora, Corrado
PY - 2000
Y1 - 2000
N2 - Exogenous DNA molecules are spontaneously taken up by sperm cells, internalized in nuclei, and eventually integrated in the sperm genome. The actual occurrence of the integration suggests that the sperm chromosomal DNA is not uniformly and tightly packed with protamines, implying the existence of genomic sites where the chromosomal DNA is accessible to foreign molecules. We have characterized a hypersensitive, nucleosomal subfraction of mouse sperm chromatin that is highly enriched in unmethylated retroposon DNA from a variety of families. Here we propose that both the integration of exogenous DNA molecules, and the endogenous retroposition activity, occur in the same site(s) of sperm chromatin. (C) 2000 Wiley-Liss, Inc.
AB - Exogenous DNA molecules are spontaneously taken up by sperm cells, internalized in nuclei, and eventually integrated in the sperm genome. The actual occurrence of the integration suggests that the sperm chromosomal DNA is not uniformly and tightly packed with protamines, implying the existence of genomic sites where the chromosomal DNA is accessible to foreign molecules. We have characterized a hypersensitive, nucleosomal subfraction of mouse sperm chromatin that is highly enriched in unmethylated retroposon DNA from a variety of families. Here we propose that both the integration of exogenous DNA molecules, and the endogenous retroposition activity, occur in the same site(s) of sperm chromatin. (C) 2000 Wiley-Liss, Inc.
KW - Nuclease hypersensitivity
KW - Nucleosomes
KW - Retroposon DNA
KW - Sperm cell chromatin
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UR - http://www.scopus.com/inward/citedby.url?scp=0034064336&partnerID=8YFLogxK
M3 - Article
VL - 56
SP - 248
EP - 251
JO - Molecular Reproduction and Development
JF - Molecular Reproduction and Development
SN - 1040-452X
IS - 2 SUPPL.
ER -