Nucleotide sequences of Dictyostelium discoideum developmentally regulated cDNAs rich in (AAC) imply proteins that contain clusters of asparagine, glutamine, or threonine

David R. Shaw, Hans Richter, Roberto Giorda, Tetsuo Ohmachi, Herbert L. Ennis

Research output: Contribution to journalArticlepeer-review

Abstract

A Dictyostelium discoideum repetitive element composed of long repeats of the codon (AAC) is found in developmentally regulated transcripts. The concentration of (AAC) sequences is low in mRNA from dormant spores and growing cells and increases markedly during spore germination and multicellular development. The sequence hybridizes to many different sized Dictyostelium DNA restriction fragments indicating that it is scattered throughout the genome. Four cDNA clones isolated contain (AAC) sequences in the deduced coding region. Interestingly, the (AAC)-rich sequences are present in all three reading frames in the deduced proteins, i.e., AAC (asparagine), ACA (threonine) and CAA (glutamine). Three of the clones contain only one of these in-frame so that the individual proteins carry either asparagine, threonine, or glutamine clusters, not mixtures. However, one clone is both glutamine- and asparagine-rich. The (AAC) portion of the transcripts are reiterated 300 times in the haploid genome while the other portions of the cDNAs represent single copy genes, whose sequences show no similarity other than the (AAC) repeats. The repeated sequence is similar to the opa or M sequence found in Drosophila melanogaster notch and homeo box genes and in fly developmentally regulated transcripts. The transcripts are present on polysomes suggesting that they are translated. Although the function of these repeats is unknown, long amino acid repeats are a characteristic feature of extracellular proteins of lower eukaryotes.

Original languageEnglish
Pages (from-to)453-459
Number of pages7
JournalMGG Molecular & General Genetics
Volume218
Issue number3
DOIs
Publication statusPublished - Sep 1989

Keywords

  • cDNAs containing (AAC) clusters
  • D. discoideum cDNAs
  • Dictyostelium discoideum developmental proteins
  • Proteins rich in Asp, Glu or Thr

ASJC Scopus subject areas

  • Genetics

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