Number of pericryptal fibroblasts correlates with density of distinct mast cell phenotypes in the crypt lamina propria of human duodenum: Implications for the homeostasis of villous architecture

Enrico Crivellato, Nicoletta Finato, Miriam Isola, Maura Pandolfi, Domenico Ribatti, Carlo Alberto Beltrami

Research output: Contribution to journalArticle

Abstract

Pericryptal fibroblasts (PFs), a class of myofibroblasts, have strongly been implicated in the regulation of villous structure because of their location close to crypts and their ability to secrete cytokines affecting intestinal epithelial cell proliferation and differentiation. Recently, mast cells (MCs) have also been involved in the homeostasis of villous architecture. As myofibroblasts arise in a wide variety of settings concurrently with a local increase in the number of tissue MCs, we calculated in this study the density of both PF and distinct pericryptal MC phenotypes in the mucosa of human duodenum showing normal, defective, or atrophic villous profiles. In addition, we evaluated the statistical association between PF-MC densities and each pattern of villous architecture. Finally, we correlated the density of PF with the density of pericryptal MC phenotypes. For this purpose, samples taken by endoscopy from 30 patients complaining of inflammatory bowel disorders were studied by immunonistochemistry. The densities of α-smooth muscle actin-positive PFs as well as tryptase-, chymase-, and c-kit-positive MCs were determined in the crypt lamina propria. Villous architecture was found to be significantly associated with the number of PFs and tryptase-, chymase-, c-kit-positive MCs in the lamina propria (ANOVA group effect P <0.001). High density of both PFs and MCs was found in intestinal samples with normal villous morphology while lower densities were associated with defective or atrophic villous profiles (Tukey's test for multiple comparison P <0.001). In addition, a significant correlation was found between PF density and the density of each pericryptal MC phenotype (vs. tryptase-positive MCs, r = 0.913; vs. chymase-positive MC, r = 0.905; vs. c-kit-positive MC, r = 0.927; P <0.001 in all cases). This study provides morphological support for an important cooperation between PFs and MCs in maintaining normal villous architecture.

Original languageEnglish
Pages (from-to)593-600
Number of pages8
JournalAnatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology
Volume288
Issue number6
DOIs
Publication statusPublished - Jun 2006

Fingerprint

laminae (animals)
homeostasis
mast cells
duodenum
Duodenum
Mast Cells
fibroblasts
phenotype
Mucous Membrane
Homeostasis
Fibroblasts
Phenotype
Chymases
tryptase
Tryptases
Myofibroblasts
group effect
muscle
endoscopy
Endoscopy

Keywords

  • Histochemistry
  • Human duodenum
  • Mast cells
  • Pericryptal fibroblasts
  • Villous architecture

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Number of pericryptal fibroblasts correlates with density of distinct mast cell phenotypes in the crypt lamina propria of human duodenum : Implications for the homeostasis of villous architecture. / Crivellato, Enrico; Finato, Nicoletta; Isola, Miriam; Pandolfi, Maura; Ribatti, Domenico; Beltrami, Carlo Alberto.

In: Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology, Vol. 288, No. 6, 06.2006, p. 593-600.

Research output: Contribution to journalArticle

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