Nutrients intake is associated with DNA methylation of candidate inflammatory genes in a population of obese subjects

Valentina Bollati, Chiara Favero, Benedetta Albetti, Letizia Tarantini, Alice Moroni, Hyang Min Byun, Valeria Motta, Diana Misaela Conti, Amedea Silvia Tirelli, Luisella Vigna, Pier Alberto Bertazzi, Angela Cecilia Pesatori

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of the present study was to evaluate the potential association between dietary nutrients and alterations in DNA methylation in a set of five candidate genes, including CD14, Et-1, iNOS, HERV-w and TNFα, in a population of overweight/obese subjects. We evaluated possible associations between gene methylation and clinical blood parameters, including total cholesterol (TC), low- and high-density lipoprotein cholesterol (LDL-C and HDL-C), triglyceride and homocysteine levels. We employed validated methods to assess anthropometric, clinical and dietary data, as well as pyrosequencing to evaluate DNA methylation of the five candidate genes in 165 overweight/obese subjects. There was no association between body mass index and DNA methylation of the five candidate genes in this group of subjects. Positive associations were observed between TNFα methylation and blood levels of LDL-C (β = 0.447, p = 0.002), TC/HDL-C (β = 0.467, p = 0.001) and LDL-C/HDL-C (β = 0.445, p = 0.002), as well as between HERV-w methylation and dietary intakes of β-carotene (β = 0.088, p = 0.051) and carotenoids (β = 0.083, p = 0.029). TNFα methylation showed negative associations with dietary intakes of cholesterol (β = −0.278, p = 0.048), folic acid (β = −0.339, p = 0.012), β-carotene (β = −0.332, p = 0.045), carotenoids (β = −0.331, p = 0.015) and retinol (β = −0.360, p = 0.008). These results suggest a complex relationship among nutrient intake, oxidative stress and DNA methylation.

Original languageEnglish
Pages (from-to)4625-4639
Number of pages15
JournalNutrients
Volume6
Issue number10
DOIs
Publication statusPublished - Oct 22 2014

Keywords

  • CD14
  • DNA methylation
  • Et-1
  • HERV-w
  • INOS
  • Nutrients
  • TNFα

ASJC Scopus subject areas

  • Food Science
  • Medicine(all)

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