Nutritional supplementation with mixed essential amino acids enhances myocyte survival, preserving mitochondrial functional capacity during ischemia-reperfusion injury

Tiziano M. Scarabelli, Evasio Pasini, Anastasis Stephanou, Carol Chen-Scarabelli, Louis Saravolatz, Richard A. Knight, David S. Latchman, Julius M. Gardin

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Abstract

In patients undergoing coronary surgery, the uptake of amino acids, which has been shown to correlate with oxygen consumption, is a mechanism of cardiac adaptation to the iatrogenic ischemia-reperfusion injury associated with cardioplegic arrest. Based on these premises, we sought to determine whether oral supplementation with mixed amino acids may protect the rat heart exposed to ischemia-reperfusion and to address whether this hypothesized cardioprotection is achieved, at least in part, through preservation of the energy-producing properties of mitochondria. Sprague-Dawley rats were fed (by enteral route) a liquid diet, with or without mixed essential amino acids (daily dose of 1 g/kg) for 30 days. Hearts from anesthetized rats were perfused by the Langendorff method and randomized to 3 groups. The control group was perfused with buffer for 60 minutes; the ischemia-reperfusion control and the amino acid-treated groups were exposed to 35 minutes of ischemia, followed by 60 or 120 minutes of reperfusion. Amino acid supplements minimized infarct size (22 ± 1.8% vs 33 ± 2.5%; p 75% in control hearts after 2 hours of reperfusion (p

Original languageEnglish
JournalThe American Journal of Cardiology
Volume93
Issue number8 SUPPL. 1
DOIs
Publication statusPublished - Apr 22 2004

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ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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