O-β-N-acetyl-D-glucosaminidase in erythrocytes of Italian air force acrobatic pilots

Massimiliano M. Corsi, Luca Massaccesi, Giada Dogliotti, Elena Vianello, Marco Agrifoglio, Fabrizio Palumbo, Giancarlo Goi

Research output: Contribution to journalArticle

Abstract

Background: Italian air force acrobatic pilots are occupationally susceptible to oxidative stress damage that can lead to overt signs and symptoms of hypoxia. We propose erythrocyte glycohydrolases as new, sensitive markers to assess oxidative stress. Methods: We measured erythrocyte concentrations of β-D-glucuronidase (GCR), hexosaminidase, O-β-N-acetyl-D- glucosaminidase (O-GlcNAcase), plasma membrane fluidity and plasma hydroperoxides from 19 pilots and compared these to 40 matched healthy subjects. Results: Plasma hydroperoxide concentrations and the erythrocyte ghosts' fluorescence anisotropy were significantly lower in the pilots. Concentrations of GCR, O-GlcNAcase and hexosaminidase in pilots were significantly different from controls, being lower, higher and higher, respectively. Conclusions: Pilots, in spite of their oxidative stress, are better protected than controls, probably as a result of their physical training and proper diet. Our results confirm that erythrocytes, with their 120-day life span, are a useful model for investigating physiopathological conditions, and glycohydrolases are good markers for monitoring oxidative stress, even in healthy people.

Original languageEnglish
Pages (from-to)213-216
Number of pages4
JournalClinical Chemistry and Laboratory Medicine
Volume48
Issue number2
DOIs
Publication statusPublished - Feb 1 2010

Fingerprint

Hexosaminidases
Oxidative stress
Erythrocytes
Air
Oxidative Stress
Glycoside Hydrolases
Glucuronidase
Hydrogen Peroxide
Plasmas
Fluidity
Cell membranes
Nutrition
Membrane Fluidity
Fluorescence Polarization
Erythrocyte Membrane
Anisotropy
Fluorescence
Signs and Symptoms
Healthy Volunteers
Cell Membrane

Keywords

  • Aircraft pilots
  • Erythrocytes
  • O-β-N-acetyl-D- glucosaminidase
  • Oxidative stress

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

O-β-N-acetyl-D-glucosaminidase in erythrocytes of Italian air force acrobatic pilots. / Corsi, Massimiliano M.; Massaccesi, Luca; Dogliotti, Giada; Vianello, Elena; Agrifoglio, Marco; Palumbo, Fabrizio; Goi, Giancarlo.

In: Clinical Chemistry and Laboratory Medicine, Vol. 48, No. 2, 01.02.2010, p. 213-216.

Research output: Contribution to journalArticle

Corsi, Massimiliano M. ; Massaccesi, Luca ; Dogliotti, Giada ; Vianello, Elena ; Agrifoglio, Marco ; Palumbo, Fabrizio ; Goi, Giancarlo. / O-β-N-acetyl-D-glucosaminidase in erythrocytes of Italian air force acrobatic pilots. In: Clinical Chemistry and Laboratory Medicine. 2010 ; Vol. 48, No. 2. pp. 213-216.
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