Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 158-167 |
| Number of pages | 10 |
| Journal | Liver International |
| Volume | 39 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2019 |
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Keywords
- hepatocellular adenoma
- hepatocyte transporters
- hepatospecific magnetic resonance imaging
- radio-pathological correlation
- b3 protein
- beta catenin
- gadolinium
- multidrug resistance associated protein 2
- multidrug resistance associated protein 3
- oatpb1 protein
- pentetic acid
- protein
- unclassified drug
- adult
- Article
- chronic liver disease
- fatty liver
- female
- human
- human tissue
- immunohistochemistry
- liver adenoma
- liver cirrhosis
- liver fibrosis
- major clinical study
- male
- malignant transformation
- medical history
- nuclear magnetic resonance imaging
- oral contraception
- protein expression
- sensitivity and specificity
Cite this
OATPB1/B3 and MRP3 expression in hepatocellular adenoma predicts Gd-EOB-DTPA uptake and correlates with risk of malignancy. / Sciarra, A.; Schmidt, S.; Pellegrinelli, A.; Maggioni, M.; Dondossola, D.; Pasquier, J.; Cigala, C.; Tosi, D.; Halkic, N.; Bulfamante, G.; Viale, G.; Bosari, S.; Balabaud, C.; Bioulac-Sage, P.; Sempoux, C.
In: Liver International, Vol. 39, No. 1, 2019, p. 158-167.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - OATPB1/B3 and MRP3 expression in hepatocellular adenoma predicts Gd-EOB-DTPA uptake and correlates with risk of malignancy
AU - Sciarra, A.
AU - Schmidt, S.
AU - Pellegrinelli, A.
AU - Maggioni, M.
AU - Dondossola, D.
AU - Pasquier, J.
AU - Cigala, C.
AU - Tosi, D.
AU - Halkic, N.
AU - Bulfamante, G.
AU - Viale, G.
AU - Bosari, S.
AU - Balabaud, C.
AU - Bioulac-Sage, P.
AU - Sempoux, C.
N1 - Export Date: 6 February 2019 CODEN: LIINC Correspondence Address: Sempoux, C.; Service of Clinical Pathology, Institute of Pathology, Lausanne University HospitalSwitzerland; email: christine.sempoux@chuv.ch Chemicals/CAS: gadolinium, 7440-54-2; multidrug resistance associated protein 2, 256503-65-8; pentetic acid, 14047-41-7, 67-43-6; protein, 67254-75-5 References: Roncalli, M., Sciarra, A., Tommaso, L.D., Benign hepatocellular nodules of healthy liver: focal nodular hyperplasia and hepatocellular adenoma (2016) Clin Mol Hepatol, 22 (2), pp. 199-211; Sempoux, C., Paradis, V., Komuta, M., Hepatocellular nodules expressing markers of hepatocellular adenomas in Budd-Chiari syndrome and other rare hepatic vascular disorders (2015) J Hepatol, 63 (5), pp. 1173-1180; Dokmak, S., Paradis, V., Vilgrain, V., A single-center surgical experience of 122 patients with single and multiple hepatocellular adenomas (2009) Gastroenterology, 137 (5), pp. 1698-1705; Chang, C.Y., Hernandez-Prera, J.C., Roayaie, S., Schwartz, M., Thung, S.N., Changing epidemiology of hepatocellular adenoma in the United States: review of the literature (2013) Int J Hepatol, 2013, p. 604860; Paradis, V., Laurent, A., Flejou, J.F., Vidaud, M., Bedossa, P., Evidence for the polyclonal nature of focal nodular hyperplasia of the liver by the study of X-chromosome inactivation (1997) Hepatology, 26 (4), pp. 891-895; Chen, Y.J., Chen, P.J., Lee, M.C., Yeh, S.H., Hsu, M.T., Lin, C.H., Chromosomal analysis of hepatic adenoma and focal nodular hyperplasia by comparative genomic hybridization (2002) Genes Chromosomes Cancer, 35 (2), pp. 138-143; Zucman-Rossi, J., Jeannot, E., Nhieu, J.T., Genotype-phenotype correlation in hepatocellular adenoma: new classification and relationship with HCC (2006) Hepatology, 43 (3), pp. 515-524; Bioulac-Sage, P., Rebouissou, S., Thomas, C., Hepatocellular adenoma subtype classification using molecular markers and immunohistochemistry (2007) Hepatology, 46 (3), pp. 740-748; Sasaki, M., Yoneda, N., Kitamura, S., Sato, Y., Nakanuma, Y., Characterization of hepatocellular adenoma based on the phenotypic classification: The Kanazawa experience (2011) Hepatol Res, 41 (10), pp. 982-988; Bellamy, C.O., Maxwell, R.S., Prost, S., Azodo, I.A., Powell, J.J., Manning, J.R., The value of immunophenotyping hepatocellular adenomas: consecutive resections at one UK centre (2013) Histopathology, 62 (3), pp. 431-445; Bioulac-Sage, P., Balabaud, C., Bedossa, P., Pathological diagnosis of liver cell adenoma and focal nodular hyperplasia: Bordeaux update (2007) J Hepatol, 46 (3), pp. 521-527; Nault, J.C., Couchy, G., Balabaud, C., Molecular classification of hepatocellular adenoma associates with risk factors, bleeding, and malignant transformation (2017) Gastroenterology, 152 (4), pp. 880-894.e886; Henriet, E., Abou Hammoud, A., Dupuy, J.W., Argininosuccinate synthase 1 (ASS1): A marker of unclassified hepatocellular adenoma and high bleeding risk (2017) Hepatology, 66 (6), pp. 2016-2028; Bioulac-Sage, P., Laumonier, H., Couchy, G., Hepatocellular adenoma management and phenotypic classification: the Bordeaux experience (2009) Hepatology, 50 (2), pp. 481-489; Cho, S.W., Marsh, J.W., Steel, J., Surgical management of hepatocellular adenoma: take it or leave it? 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PY - 2019
Y1 - 2019
N2 - Background and Aims: Hepatobiliary phase (HBP) Gd-EOB-DTPA-enhanced magnetic resonance imaging (MRI) has increased the accuracy in differentiating focal nodular hyperplasia (FNH) and hepatocellular adenoma (HCA). However, the ability of this technique to distinguish HCA subtypes remains controversial. The aim of this study was to investigate the expression of hepatocyte transporters (OATPB1/B3, MRP2, MRP3) in HCA subtypes, hence to understand their MRI signal intensity on HBP Gd-EOB-DTPA-enhanced MRI. Methods: By means of immunohistochemistry (IHC), we scored the expression of OATPB1/B3, MRP2 and MRP3, in resected specimens of FNH (n = 40), subtyped HCA (n = 58) and HCA with focal malignant transformation (HCA-HCC, n = 4). Results were validated on a supplementary set of FNH (n = 6), subtyped HCA (n = 17) and HCA-HCC (n = 1) with Gd-EOB-DTPA MR images. Results: All FNH showed a preserved expression of hepatocytes transporters. Beta-catenin-activated HCA (at highest risk of malignant transformation) and HCA-HCC were characterized by preserved/increased OATPB1/B3 expression (predictor of hyperintensity on HBP), as opposed to other HCA subtypes (P
AB - Background and Aims: Hepatobiliary phase (HBP) Gd-EOB-DTPA-enhanced magnetic resonance imaging (MRI) has increased the accuracy in differentiating focal nodular hyperplasia (FNH) and hepatocellular adenoma (HCA). However, the ability of this technique to distinguish HCA subtypes remains controversial. The aim of this study was to investigate the expression of hepatocyte transporters (OATPB1/B3, MRP2, MRP3) in HCA subtypes, hence to understand their MRI signal intensity on HBP Gd-EOB-DTPA-enhanced MRI. Methods: By means of immunohistochemistry (IHC), we scored the expression of OATPB1/B3, MRP2 and MRP3, in resected specimens of FNH (n = 40), subtyped HCA (n = 58) and HCA with focal malignant transformation (HCA-HCC, n = 4). Results were validated on a supplementary set of FNH (n = 6), subtyped HCA (n = 17) and HCA-HCC (n = 1) with Gd-EOB-DTPA MR images. Results: All FNH showed a preserved expression of hepatocytes transporters. Beta-catenin-activated HCA (at highest risk of malignant transformation) and HCA-HCC were characterized by preserved/increased OATPB1/B3 expression (predictor of hyperintensity on HBP), as opposed to other HCA subtypes (P
KW - hepatocellular adenoma
KW - hepatocyte transporters
KW - hepatospecific magnetic resonance imaging
KW - radio-pathological correlation
KW - b3 protein
KW - beta catenin
KW - gadolinium
KW - multidrug resistance associated protein 2
KW - multidrug resistance associated protein 3
KW - oatpb1 protein
KW - pentetic acid
KW - protein
KW - unclassified drug
KW - adult
KW - Article
KW - chronic liver disease
KW - fatty liver
KW - female
KW - human
KW - human tissue
KW - immunohistochemistry
KW - liver adenoma
KW - liver cirrhosis
KW - liver fibrosis
KW - major clinical study
KW - male
KW - malignant transformation
KW - medical history
KW - nuclear magnetic resonance imaging
KW - oral contraception
KW - protein expression
KW - sensitivity and specificity
U2 - 10.1111/liv.13964
DO - 10.1111/liv.13964
M3 - Article
VL - 39
SP - 158
EP - 167
JO - Liver International
JF - Liver International
SN - 1478-3223
IS - 1
ER -