OATPB1/B3 and MRP3 expression in hepatocellular adenoma predicts Gd-EOB-DTPA uptake and correlates with risk of malignancy

A. Sciarra, S. Schmidt, A. Pellegrinelli, M. Maggioni, D. Dondossola, J. Pasquier, C. Cigala, D. Tosi, N. Halkic, G. Bulfamante, G. Viale, S. Bosari, C. Balabaud, P. Bioulac-Sage, C. Sempoux

Research output: Contribution to journalArticlepeer-review

Abstract

Background and Aims: Hepatobiliary phase (HBP) Gd-EOB-DTPA-enhanced magnetic resonance imaging (MRI) has increased the accuracy in differentiating focal nodular hyperplasia (FNH) and hepatocellular adenoma (HCA). However, the ability of this technique to distinguish HCA subtypes remains controversial. The aim of this study was to investigate the expression of hepatocyte transporters (OATPB1/B3, MRP2, MRP3) in HCA subtypes, hence to understand their MRI signal intensity on HBP Gd-EOB-DTPA-enhanced MRI. Methods: By means of immunohistochemistry (IHC), we scored the expression of OATPB1/B3, MRP2 and MRP3, in resected specimens of FNH (n = 40), subtyped HCA (n = 58) and HCA with focal malignant transformation (HCA-HCC, n = 4). Results were validated on a supplementary set of FNH (n = 6), subtyped HCA (n = 17) and HCA-HCC (n = 1) with Gd-EOB-DTPA MR images. Results: All FNH showed a preserved expression of hepatocytes transporters. Beta-catenin-activated HCA (at highest risk of malignant transformation) and HCA-HCC were characterized by preserved/increased OATPB1/B3 expression (predictor of hyperintensity on HBP), as opposed to other HCA subtypes (P 
Original languageEnglish
Pages (from-to)158-167
Number of pages10
JournalLiver International
Volume39
Issue number1
DOIs
Publication statusPublished - 2019

Keywords

  • hepatocellular adenoma
  • hepatocyte transporters
  • hepatospecific magnetic resonance imaging
  • radio-pathological correlation
  • b3 protein
  • beta catenin
  • gadolinium
  • multidrug resistance associated protein 2
  • multidrug resistance associated protein 3
  • oatpb1 protein
  • pentetic acid
  • protein
  • unclassified drug
  • adult
  • Article
  • chronic liver disease
  • fatty liver
  • female
  • human
  • human tissue
  • immunohistochemistry
  • liver adenoma
  • liver cirrhosis
  • liver fibrosis
  • major clinical study
  • male
  • malignant transformation
  • medical history
  • nuclear magnetic resonance imaging
  • oral contraception
  • protein expression
  • sensitivity and specificity

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