Obesity modifies expression profiles of metabolic markers in superficial and deep subcutaneous abdominal adipose tissue depots

Gillian E. Walker, Paolo Marzullo, Flavia Prodam, Gianni Bona, Anna Maria Di Blasio

Research output: Contribution to journalArticle

Abstract

While visceral adipose tissue (VAT) associates to obesity, there is debate for subcutaneous adipose tissue (SAT). One explanation may be SAT subcompartments, superficial-SAT (sSAT) and deep-SAT (dSAT), recently recognized as independent depots. Our aim was to establish roles for sSAT/dSAT with obesity by examining the expression of proteins key to adipocyte metabolism. Paired biopsies from sSAT and dSAT of 10 normal-weight (BMI 21.8 ± 0.8 kg/m2) and 11 obese subjects (BMI 44 ± 2.1 kg/m) were analyzed for differences in insulin sensitivity using adiponectin, GLUT4 and resistin, glucocorticoid metabolism by 11βHSD1 and alterations of the adipokines leptin and TNFα. Between lean and obese subjects, sSAT and dSAT changes for GLUT4, resistin and TNFα were equivalent. Resistin and TNFa increased in both obese SAT sub-compartments; 33-fold (sSAT; P <0.006) and 18.5-fold (dSAT; P <0.003) higher resistin, with undetectable in leans to significant TNFα levels in obese. In contrast, GLUT4 showed 5.5-fold (sSAT; P <0.03) and 7-fold (dSAT; P <0.03) lower levels in obese, correlating to BMI (r = -0.6423, P = 0.007) and HOMA-IR (r = -0.5882, P = 0.017). Exclusive sSAT-specific differences were observed for adiponectin, leptin, and 11 βHSD1. Both sSAT 11 βHSD1 and leptin increased in obese, with 11 βHSD1 2.5-fold (P = 0.052) and leptin 3.3-fold (P <0.008) higher, with 11βHSD1 correlating to HOMA-IR (r = 0.5203, P = 0.0323) and leptin to BMI (r = 0.5810, P = 0.01). In contrast, obese had 7-fold (P <0.02) lower sSAT adiponectin, correlating to BMI (r = -0.5178, P = 0.027) and HOMA-IR (r = -0.4570, P = 0.049). Overall, sSAT and dSAT are distinct abdominal adipose tissue depots with independent metabolic functions. Between the two, sSAT shows clear independent effects that associate to obesity and its metabolic complications.

Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalEndocrine
Volume46
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Abdominal Subcutaneous Fat
Metabolome
Subcutaneous Fat
Resistin
Leptin
Obesity
Adiponectin
Abdominal Fat
Adipokines
Intra-Abdominal Fat
Adipocytes
Glucocorticoids
Insulin Resistance
Biopsy
Weights and Measures
Proteins

Keywords

  • Adipose tissue
  • Deep subcutaneous
  • Obesity
  • Superficial subcutaneous

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Obesity modifies expression profiles of metabolic markers in superficial and deep subcutaneous abdominal adipose tissue depots. / Walker, Gillian E.; Marzullo, Paolo; Prodam, Flavia; Bona, Gianni; Di Blasio, Anna Maria.

In: Endocrine, Vol. 46, No. 1, 2014, p. 99-106.

Research output: Contribution to journalArticle

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