Observations on specific Giemsa staining of the Y and on selective oil destaining of the chromosomes

Bruno Dallapiccola, Nicola Ricci

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The human Y chromosome can be differentially stained with Giemsa using simple procedures. This phenomenon is strikingly similar to that observed with quinacrine fluorescence. The specific Giemsa-Y stain may be selectively removed by the action of an oil. The same oil, under certain conditions, selectively removes Giemsa stain from all chromosomes, resulting in R- and T-banding patterns. These bands, which are obtained through subtraction of dye from Giemsa-stained chromosomes, allow slides to be further processed.

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalHuman Genetics
Volume26
Issue number3
DOIs
Publication statusPublished - Sep 1975

Fingerprint

Azure Stains
Oils
Chromosomes
Chromosomes, Human, Y
Staining and Labeling
Quinacrine
Coloring Agents
Fluorescence
stains-all

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Medicine(all)

Cite this

Observations on specific Giemsa staining of the Y and on selective oil destaining of the chromosomes. / Dallapiccola, Bruno; Ricci, Nicola.

In: Human Genetics, Vol. 26, No. 3, 09.1975, p. 251-255.

Research output: Contribution to journalArticle

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