TY - JOUR
T1 - Occurrence of apoptosis in serosa of Periplaneta americana L. (Blattaria
T2 - Blattidae) ultrastructural and biochemical features
AU - Barni, Sergio
AU - Lambiase, Simonetta
AU - Grigolo, Aldo
AU - Sacchi, Luciano
AU - Corona, Silvia
AU - Ivana Scovassi, Anna
AU - Laudani, Ugo
PY - 1997/10
Y1 - 1997/10
N2 - In 16-17-day-old embryos of Periplaneta americana, the amnion-serosa penetrates the cavity of the middle intestine, where it forms a cluster of compressed roundish cells. We demonstrated that these cells degenerate throughout apoptosis. The programmed cell death revealed by morphological and biochemical approaches showed all the apoptotic steps: chromatin fragmentation and pyknosis, cytoplasm condensation, karyorrhexis, cytoplasm cleavage. Nevertheless, some ultrastructural peculiarities (atypical heterochromatin arrangement, appearance of nuclear envelope protrusions, absence of nucleolar structures) suggest that the apoptotic expression partially depends on the biological situation (type of organism and inducing factors) in which the programmed cell death takes place. The presence of histiocytic cells internalizing cell debris, of apoptotic and non-apototic derivation, may be correlated with the importance of recycling substances useful for embryo growth.
AB - In 16-17-day-old embryos of Periplaneta americana, the amnion-serosa penetrates the cavity of the middle intestine, where it forms a cluster of compressed roundish cells. We demonstrated that these cells degenerate throughout apoptosis. The programmed cell death revealed by morphological and biochemical approaches showed all the apoptotic steps: chromatin fragmentation and pyknosis, cytoplasm condensation, karyorrhexis, cytoplasm cleavage. Nevertheless, some ultrastructural peculiarities (atypical heterochromatin arrangement, appearance of nuclear envelope protrusions, absence of nucleolar structures) suggest that the apoptotic expression partially depends on the biological situation (type of organism and inducing factors) in which the programmed cell death takes place. The presence of histiocytic cells internalizing cell debris, of apoptotic and non-apototic derivation, may be correlated with the importance of recycling substances useful for embryo growth.
KW - Apoptosis
KW - Dorsal organ
KW - Embryogenesis
KW - Periplaneta americana
KW - Serosa
UR - http://www.scopus.com/inward/record.url?scp=0031253035&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031253035&partnerID=8YFLogxK
U2 - 10.1016/S0022-1910(97)00075-9
DO - 10.1016/S0022-1910(97)00075-9
M3 - Article
AN - SCOPUS:0031253035
VL - 43
SP - 999
EP - 1008
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
SN - 0022-1910
IS - 11
ER -