Oct-4 expression in adult human differentiated cells challenges its role as a pure stem cell marker

Stefano Zangrossi, Mirko Marabese, Massimo Broggini, Rosaria Giordano, Marco D'Erasmo, Elisa Montelatici, Daniela Intini, Antonino Neri, Maurizio Pesce, Paolo Rebulla, Lorenza Lazzari

Research output: Contribution to journalArticlepeer-review

Abstract

The Oct-4 transcription factor, a member of the POU family that is also known as Oct-3 and Oct3/4, is expressed in totipotent embryonic stem cells (ES) and germ cells, and it has a unique role in development and in the determination of pluripotency. ES may have their postnatal counterpart in the adult stem cells, recently described in various mammalian tissues, and Oct-4 expression in putative stem cells purified from adult tissues has been considered a real marker of stemness. In this context, normal mature adult cells would not be expected to show Oct-4 expression. On the contrary, we demonstrated, using reverse transcription-polymerase chain reaction (PCR) (total RNA, Poly A+), real-time PCR, immunoprecipitation, Western blotting, band shift, and immunofluorescence, that human peripheral blood mononuclear cells, genetically stable and mainly terminally differentiated cells with well defined functions and a limited lifespan, express Oct-4. These observations raise the question as to whether the role of Oct-4 as a marker of pluripotency should be challenged. Our findings suggest that the presence of Oct-4 is not sufficient to define a cell as pluripotent, and that additional measures should be used to avoid misleading results in the case of an embryonic-specific gene with a large number of pseudogenes that may contribute to false identification of Oct-4 in adult stem cells. These unexpected findings may provide new insights into the role of Oct-4 in fully differentiated cells.

Original languageEnglish
Pages (from-to)1675-1680
Number of pages6
JournalStem Cells
Volume25
Issue number7
DOIs
Publication statusPublished - Jul 2007

Keywords

  • Human peripheral cells
  • Oct-4
  • Stem cell marker

ASJC Scopus subject areas

  • Cell Biology

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