Oct4 is required for primordial germ cell survival

James Kehler, Elena Tolkunova, Birgit Koschorz, Maurizio Pesce, Luca Gentile, Michele Boiani, Hilda Lomelí, Andras Nagy, K. John McLaughlin, Hans R. Schöler, Alexey Tomilin

Research output: Contribution to journalArticle

405 Citations (Scopus)

Abstract

Previous studies have shown that Oct4 has an essential role in maintaining pluripotency of cells of the inner cell mass (ICM) and embryonic stem cells. However, Oct4 null homozygous embryos die around the time of implantation, thus precluding further analysis of gene function during development. We have used the conditional Cre/loxP gene targeting strategy to assess Oct4 function in primordial germ cells (PGCs). Loss of Oct4 function leads to apoptosis of PGCs rather than to differentiation into a trophectodermal lineage, as has been described for Oct4-deficient ICM cells. These new results suggest a previously unknown function of Oct4 in maintaining viability of mammalian germline.

Original languageEnglish
Pages (from-to)1078-1083
Number of pages6
JournalEMBO Reports
Volume5
Issue number11
DOIs
Publication statusPublished - Nov 2004

Fingerprint

Germ Cells
Cell Survival
Cells
Genes
Gene Targeting
Embryonic Stem Cells
Stem cells
Embryonic Structures
Apoptosis

Keywords

  • Apoptosis
  • Conditional gene targeting
  • Cre/IoxP
  • Oct4 function
  • Primordial germ cells

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Kehler, J., Tolkunova, E., Koschorz, B., Pesce, M., Gentile, L., Boiani, M., ... Tomilin, A. (2004). Oct4 is required for primordial germ cell survival. EMBO Reports, 5(11), 1078-1083. https://doi.org/10.1038/sj.embor.7400279

Oct4 is required for primordial germ cell survival. / Kehler, James; Tolkunova, Elena; Koschorz, Birgit; Pesce, Maurizio; Gentile, Luca; Boiani, Michele; Lomelí, Hilda; Nagy, Andras; McLaughlin, K. John; Schöler, Hans R.; Tomilin, Alexey.

In: EMBO Reports, Vol. 5, No. 11, 11.2004, p. 1078-1083.

Research output: Contribution to journalArticle

Kehler, J, Tolkunova, E, Koschorz, B, Pesce, M, Gentile, L, Boiani, M, Lomelí, H, Nagy, A, McLaughlin, KJ, Schöler, HR & Tomilin, A 2004, 'Oct4 is required for primordial germ cell survival', EMBO Reports, vol. 5, no. 11, pp. 1078-1083. https://doi.org/10.1038/sj.embor.7400279
Kehler J, Tolkunova E, Koschorz B, Pesce M, Gentile L, Boiani M et al. Oct4 is required for primordial germ cell survival. EMBO Reports. 2004 Nov;5(11):1078-1083. https://doi.org/10.1038/sj.embor.7400279
Kehler, James ; Tolkunova, Elena ; Koschorz, Birgit ; Pesce, Maurizio ; Gentile, Luca ; Boiani, Michele ; Lomelí, Hilda ; Nagy, Andras ; McLaughlin, K. John ; Schöler, Hans R. ; Tomilin, Alexey. / Oct4 is required for primordial germ cell survival. In: EMBO Reports. 2004 ; Vol. 5, No. 11. pp. 1078-1083.
@article{014d189509074dd2870c894139fefb2b,
title = "Oct4 is required for primordial germ cell survival",
abstract = "Previous studies have shown that Oct4 has an essential role in maintaining pluripotency of cells of the inner cell mass (ICM) and embryonic stem cells. However, Oct4 null homozygous embryos die around the time of implantation, thus precluding further analysis of gene function during development. We have used the conditional Cre/loxP gene targeting strategy to assess Oct4 function in primordial germ cells (PGCs). Loss of Oct4 function leads to apoptosis of PGCs rather than to differentiation into a trophectodermal lineage, as has been described for Oct4-deficient ICM cells. These new results suggest a previously unknown function of Oct4 in maintaining viability of mammalian germline.",
keywords = "Apoptosis, Conditional gene targeting, Cre/IoxP, Oct4 function, Primordial germ cells",
author = "James Kehler and Elena Tolkunova and Birgit Koschorz and Maurizio Pesce and Luca Gentile and Michele Boiani and Hilda Lomel{\'i} and Andras Nagy and McLaughlin, {K. John} and Sch{\"o}ler, {Hans R.} and Alexey Tomilin",
year = "2004",
month = "11",
doi = "10.1038/sj.embor.7400279",
language = "English",
volume = "5",
pages = "1078--1083",
journal = "EMBO Reports",
issn = "1469-221X",
publisher = "Wiley-VCH Verlag GmbH",
number = "11",

}

TY - JOUR

T1 - Oct4 is required for primordial germ cell survival

AU - Kehler, James

AU - Tolkunova, Elena

AU - Koschorz, Birgit

AU - Pesce, Maurizio

AU - Gentile, Luca

AU - Boiani, Michele

AU - Lomelí, Hilda

AU - Nagy, Andras

AU - McLaughlin, K. John

AU - Schöler, Hans R.

AU - Tomilin, Alexey

PY - 2004/11

Y1 - 2004/11

N2 - Previous studies have shown that Oct4 has an essential role in maintaining pluripotency of cells of the inner cell mass (ICM) and embryonic stem cells. However, Oct4 null homozygous embryos die around the time of implantation, thus precluding further analysis of gene function during development. We have used the conditional Cre/loxP gene targeting strategy to assess Oct4 function in primordial germ cells (PGCs). Loss of Oct4 function leads to apoptosis of PGCs rather than to differentiation into a trophectodermal lineage, as has been described for Oct4-deficient ICM cells. These new results suggest a previously unknown function of Oct4 in maintaining viability of mammalian germline.

AB - Previous studies have shown that Oct4 has an essential role in maintaining pluripotency of cells of the inner cell mass (ICM) and embryonic stem cells. However, Oct4 null homozygous embryos die around the time of implantation, thus precluding further analysis of gene function during development. We have used the conditional Cre/loxP gene targeting strategy to assess Oct4 function in primordial germ cells (PGCs). Loss of Oct4 function leads to apoptosis of PGCs rather than to differentiation into a trophectodermal lineage, as has been described for Oct4-deficient ICM cells. These new results suggest a previously unknown function of Oct4 in maintaining viability of mammalian germline.

KW - Apoptosis

KW - Conditional gene targeting

KW - Cre/IoxP

KW - Oct4 function

KW - Primordial germ cells

UR - http://www.scopus.com/inward/record.url?scp=10044285191&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10044285191&partnerID=8YFLogxK

U2 - 10.1038/sj.embor.7400279

DO - 10.1038/sj.embor.7400279

M3 - Article

C2 - 15486564

AN - SCOPUS:10044285191

VL - 5

SP - 1078

EP - 1083

JO - EMBO Reports

JF - EMBO Reports

SN - 1469-221X

IS - 11

ER -