Oleamide-mediated sleep induction does not depend on perturbation of membrane homeoviscosity

Marco Gobbi, Tiziana Mennini, Fabio Dalla Valle, Luigi Cervo, Mario Salmona, Luisa Diomede

Research output: Contribution to journalArticle

Abstract

To verify whether the sleep-inducing properties of oleamide were related to its ability to perturb membrane homeoviscosity, affecting 5-HT(2A) receptors, we compared the effects of oleamide and oleic acid, the latter lacking both the sleep-inducing effect and the action on 5-HT(2A) receptors. In binding studies the two compounds did not directly interact with rat brain cortex 5-HT(2A) receptors, nor did they increase the affinity of a 5-HT(2A) agonist, either in vitro or ex vivo. They had similar fluidizing effects, in vitro at high concentrations (≥10 μM), and ex vivo after a dose of 100 mg/kg, and they reduced locomotor activity with similar potency. There thus appears to be no causal relationship between the fluidizing effects of oleamide and its sleep-inducing properties.

Original languageEnglish
Pages (from-to)281-284
Number of pages4
JournalFEBS Letters
Volume463
Issue number3
DOIs
Publication statusPublished - Dec 17 1999

Keywords

  • 5-HT(2A) receptor
  • Membrane homeoviscosity
  • Oleamide
  • Oleic acid
  • Rat
  • Sleep

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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