Olfactory receptors in semen and in the male tract: From proteome to proteins

Domenico Milardi, Claudia Colussi, Giuseppe Grande, Federica Vincenzoni, Francesco Pierconti, Francesca Mancini, Silvia Baroni, Massimo Castagnola, Riccardo Marana, Alfredo Pontecorvi

Research output: Contribution to journalArticle

Abstract

The estimated number of testicular olfactory receptors (ORs) in mammals range between 20 and 66. Previous data reported the role of hOR17-4 and mOR23 in sperm-oocyte chemiotaxis. Proteomic analysis was performed to understand which are the ORs expressed in seminal plasma. Seminal samples by four fertile men were analyzed by an Ultimate 3000Nano/Micro-HPLC apparatus coupled with an LTQ-Orbitrap XL hybrid mass spectrometer. Western blot analysis confirmed the expression of three identified ORs. The expression of ORs in sperm cells, testis, and epididymis was evaluated by confocal microscopy analysis. In seminal plasma eight different ORs were identified by proteomics and three ORs have been confirmed by western blot. Confocal microscopy analysis revealed that OR4S1, OR4C13, and OR1I1 are expressed on the surface of sperm cells. In testicular tissue, OR4S1 and OR1I1 are expressed in spermatocytes and spermatids and OR4C13 is expressed throughout all the tubules. In patients with spermatocyte maturation arrest OR4S1 and OR1I1 expression was reduced and a weak positivity for OR4C13 was detected in the spermatogonia. OR4S1, OR4C13, and OR1I1 had intense and diffuse staining in the epididymis. This study initiated a new methodology for screening OR repertoire in sperms, testis and epididymis. Our results open new insights into OR involvement in sperm maturation and migration.

Original languageEnglish
Article number379
JournalFrontiers in Endocrinology
Volume8
Issue numberJAN
DOIs
Publication statusPublished - Jan 23 2018

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Odorant Receptors
Proteome
Semen
Spermatozoa
Epididymis
Proteins
Spermatocytes
Confocal Microscopy
Proteomics
Testis
Western Blotting
Sperm Maturation
Spermatogonia
Spermatids
Oocytes
Mammals
High Pressure Liquid Chromatography
Staining and Labeling

Keywords

  • Olfactory receptors
  • OR1I
  • OR4C13
  • OR4S1
  • Sperm
  • Spermatogenesis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

Cite this

Olfactory receptors in semen and in the male tract : From proteome to proteins. / Milardi, Domenico; Colussi, Claudia; Grande, Giuseppe; Vincenzoni, Federica; Pierconti, Francesco; Mancini, Francesca; Baroni, Silvia; Castagnola, Massimo; Marana, Riccardo; Pontecorvi, Alfredo.

In: Frontiers in Endocrinology, Vol. 8, No. JAN, 379, 23.01.2018.

Research output: Contribution to journalArticle

Milardi, D, Colussi, C, Grande, G, Vincenzoni, F, Pierconti, F, Mancini, F, Baroni, S, Castagnola, M, Marana, R & Pontecorvi, A 2018, 'Olfactory receptors in semen and in the male tract: From proteome to proteins', Frontiers in Endocrinology, vol. 8, no. JAN, 379. https://doi.org/10.3389/fendo.2017.00379
Milardi, Domenico ; Colussi, Claudia ; Grande, Giuseppe ; Vincenzoni, Federica ; Pierconti, Francesco ; Mancini, Francesca ; Baroni, Silvia ; Castagnola, Massimo ; Marana, Riccardo ; Pontecorvi, Alfredo. / Olfactory receptors in semen and in the male tract : From proteome to proteins. In: Frontiers in Endocrinology. 2018 ; Vol. 8, No. JAN.
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