Oligoclonality of lung T lymphocytes following exposure to allergen in asthma

S. E. Burastero, E. Crimi, A. Balbo, M. Vavassori, B. Borgonovo, D. Gaffi, E. Frittoli, G. Casorati, G. A. Rossi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We were interested in studing the lung allergen-specific T cell repertoire in different conditions of allergen exposure in subjects with atopic asthma. Twenty-one allergic individuals were studied: 17 subjects suffering mainly from asthma and 4 from rhinitis. They all performed spirometry and methacholine challenge. All patients were subjected to bronchoalveolar lavage (BAL), either at base line (no challenge) or after allergen or histamine challenge, and the TCR repertoire of their lung T cells was studied with heteroduplex analysis. Expansion of single T cell clones was observed in one (of seven) asthmatic subject that reported a recent exposure to allergen and had high bronchial hypersensitivity to methacholine, and in seven/seven asthmatic subjects who underwent BAL after they suffered an early asthmatic reaction to experimental allergen inhalation. Remarkably, ex vivo expanded clones included allergen-specific T cells. In two of the seven subjects who underwent BAL after allergen challenge, two different lung segments were lavaged. A strikingly symmetrical distribution of the expanded clones was found in these samples. Control subjects and six of seven asthmatic patients studied at base line showed polyclonality of lung T cells. In conclusion, T lymphocytes are clonally expanded in the lower respiratory tract only in asthmatic subjects exposed to allergen. These results suggest that in allergic asthma, the inhalation of sensitizing allergens can recruit to the lung T lymphocytes that include allergen-specific T cell clones.

Original languageEnglish
Pages (from-to)5836-5846
Number of pages11
JournalJournal of Immunology
Volume155
Issue number12
Publication statusPublished - 1995

Fingerprint

Allergens
Asthma
T-Lymphocytes
Lung
Bronchoalveolar Lavage
Clone Cells
Methacholine Chloride
Inhalation
Heteroduplex Analysis
Spirometry
Rhinitis
Respiratory System
Histamine
Hypersensitivity

ASJC Scopus subject areas

  • Immunology

Cite this

Burastero, S. E., Crimi, E., Balbo, A., Vavassori, M., Borgonovo, B., Gaffi, D., ... Rossi, G. A. (1995). Oligoclonality of lung T lymphocytes following exposure to allergen in asthma. Journal of Immunology, 155(12), 5836-5846.

Oligoclonality of lung T lymphocytes following exposure to allergen in asthma. / Burastero, S. E.; Crimi, E.; Balbo, A.; Vavassori, M.; Borgonovo, B.; Gaffi, D.; Frittoli, E.; Casorati, G.; Rossi, G. A.

In: Journal of Immunology, Vol. 155, No. 12, 1995, p. 5836-5846.

Research output: Contribution to journalArticle

Burastero, SE, Crimi, E, Balbo, A, Vavassori, M, Borgonovo, B, Gaffi, D, Frittoli, E, Casorati, G & Rossi, GA 1995, 'Oligoclonality of lung T lymphocytes following exposure to allergen in asthma', Journal of Immunology, vol. 155, no. 12, pp. 5836-5846.
Burastero SE, Crimi E, Balbo A, Vavassori M, Borgonovo B, Gaffi D et al. Oligoclonality of lung T lymphocytes following exposure to allergen in asthma. Journal of Immunology. 1995;155(12):5836-5846.
Burastero, S. E. ; Crimi, E. ; Balbo, A. ; Vavassori, M. ; Borgonovo, B. ; Gaffi, D. ; Frittoli, E. ; Casorati, G. ; Rossi, G. A. / Oligoclonality of lung T lymphocytes following exposure to allergen in asthma. In: Journal of Immunology. 1995 ; Vol. 155, No. 12. pp. 5836-5846.
@article{c020097629374884a03eacc45ebf4351,
title = "Oligoclonality of lung T lymphocytes following exposure to allergen in asthma",
abstract = "We were interested in studing the lung allergen-specific T cell repertoire in different conditions of allergen exposure in subjects with atopic asthma. Twenty-one allergic individuals were studied: 17 subjects suffering mainly from asthma and 4 from rhinitis. They all performed spirometry and methacholine challenge. All patients were subjected to bronchoalveolar lavage (BAL), either at base line (no challenge) or after allergen or histamine challenge, and the TCR repertoire of their lung T cells was studied with heteroduplex analysis. Expansion of single T cell clones was observed in one (of seven) asthmatic subject that reported a recent exposure to allergen and had high bronchial hypersensitivity to methacholine, and in seven/seven asthmatic subjects who underwent BAL after they suffered an early asthmatic reaction to experimental allergen inhalation. Remarkably, ex vivo expanded clones included allergen-specific T cells. In two of the seven subjects who underwent BAL after allergen challenge, two different lung segments were lavaged. A strikingly symmetrical distribution of the expanded clones was found in these samples. Control subjects and six of seven asthmatic patients studied at base line showed polyclonality of lung T cells. In conclusion, T lymphocytes are clonally expanded in the lower respiratory tract only in asthmatic subjects exposed to allergen. These results suggest that in allergic asthma, the inhalation of sensitizing allergens can recruit to the lung T lymphocytes that include allergen-specific T cell clones.",
author = "Burastero, {S. E.} and E. Crimi and A. Balbo and M. Vavassori and B. Borgonovo and D. Gaffi and E. Frittoli and G. Casorati and Rossi, {G. A.}",
year = "1995",
language = "English",
volume = "155",
pages = "5836--5846",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "12",

}

TY - JOUR

T1 - Oligoclonality of lung T lymphocytes following exposure to allergen in asthma

AU - Burastero, S. E.

AU - Crimi, E.

AU - Balbo, A.

AU - Vavassori, M.

AU - Borgonovo, B.

AU - Gaffi, D.

AU - Frittoli, E.

AU - Casorati, G.

AU - Rossi, G. A.

PY - 1995

Y1 - 1995

N2 - We were interested in studing the lung allergen-specific T cell repertoire in different conditions of allergen exposure in subjects with atopic asthma. Twenty-one allergic individuals were studied: 17 subjects suffering mainly from asthma and 4 from rhinitis. They all performed spirometry and methacholine challenge. All patients were subjected to bronchoalveolar lavage (BAL), either at base line (no challenge) or after allergen or histamine challenge, and the TCR repertoire of their lung T cells was studied with heteroduplex analysis. Expansion of single T cell clones was observed in one (of seven) asthmatic subject that reported a recent exposure to allergen and had high bronchial hypersensitivity to methacholine, and in seven/seven asthmatic subjects who underwent BAL after they suffered an early asthmatic reaction to experimental allergen inhalation. Remarkably, ex vivo expanded clones included allergen-specific T cells. In two of the seven subjects who underwent BAL after allergen challenge, two different lung segments were lavaged. A strikingly symmetrical distribution of the expanded clones was found in these samples. Control subjects and six of seven asthmatic patients studied at base line showed polyclonality of lung T cells. In conclusion, T lymphocytes are clonally expanded in the lower respiratory tract only in asthmatic subjects exposed to allergen. These results suggest that in allergic asthma, the inhalation of sensitizing allergens can recruit to the lung T lymphocytes that include allergen-specific T cell clones.

AB - We were interested in studing the lung allergen-specific T cell repertoire in different conditions of allergen exposure in subjects with atopic asthma. Twenty-one allergic individuals were studied: 17 subjects suffering mainly from asthma and 4 from rhinitis. They all performed spirometry and methacholine challenge. All patients were subjected to bronchoalveolar lavage (BAL), either at base line (no challenge) or after allergen or histamine challenge, and the TCR repertoire of their lung T cells was studied with heteroduplex analysis. Expansion of single T cell clones was observed in one (of seven) asthmatic subject that reported a recent exposure to allergen and had high bronchial hypersensitivity to methacholine, and in seven/seven asthmatic subjects who underwent BAL after they suffered an early asthmatic reaction to experimental allergen inhalation. Remarkably, ex vivo expanded clones included allergen-specific T cells. In two of the seven subjects who underwent BAL after allergen challenge, two different lung segments were lavaged. A strikingly symmetrical distribution of the expanded clones was found in these samples. Control subjects and six of seven asthmatic patients studied at base line showed polyclonality of lung T cells. In conclusion, T lymphocytes are clonally expanded in the lower respiratory tract only in asthmatic subjects exposed to allergen. These results suggest that in allergic asthma, the inhalation of sensitizing allergens can recruit to the lung T lymphocytes that include allergen-specific T cell clones.

UR - http://www.scopus.com/inward/record.url?scp=0028875547&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028875547&partnerID=8YFLogxK

M3 - Article

VL - 155

SP - 5836

EP - 5846

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 12

ER -