Omentum-derived stromal cells improve myocardial regeneration in pig post-infarcted heart through a potent paracrine mechanism

Rocco De Siena, Luigi Balducci, Antonella Blasi, Manuela Gessica Montanaro, Marilisa Saldarelli, Vittorio Saponaro, Carmela Martino, Gaetano Logrieco, Antonio Soleti, Simona Fiobellot, Paolo Madeddu, Giacomo Rossi, Domenico Ribatti, Antonio Crovace, Silvia Cristini, Gloria Invernici, Eugenio Agostino Parati, Giulio Alessandri

Research output: Contribution to journalArticle

Abstract

Cell-based therapy could be a valid option to treat myocardial infarct (MI). Adipose-derived stromal cells (ADStCs) have demonstrated tissue regenerative potential including cardiomyogenesis. Omentum is an extremely rich source of visceral fat and its accumulation seems to correlate with cardiovascular diseases. We investigated the capacity of human fat Omentum-derived StCs (FOStCs) to affect heart function upon acute infarct in pigs induced by permanent ligation of the anterior interventricular artery (IVA). We demonstrated for the first time that the local injection of 50×106 of FOStCs ameliorates the functional parameters of post-infarct heart. Most importantly, histology of FOStCs treated hearts demonstrated a substantial improvement of cardiomyogenesis. In culture, FOStCs produced an impressive number and amount of angiogenic factors and cytokines. Moreover, the conditioned medium of FOStCs (FOStCs-CM) stimulates in vitro cardiac endothelial cells (ECs) proliferation and vascular morphogenesis and inhibits monocytes, EC activation and cardiomyocyte apoptosis. Since FOStCs in vivo did not trans-differentiate into cardiomyocyte-like cells, we conclude that FOStCs efficacy was presumably mediated by a potent paracrine mechanism involving molecules that concomitantly improved angiogenesis, reduced inflammation and prevented cardiomyocytes death. Our results highlight for the first time the important role that human FOStCs may have in cardiac regeneration.

Original languageEnglish
Pages (from-to)1804-1815
Number of pages12
JournalExperimental Cell Research
Volume316
Issue number11
DOIs
Publication statusPublished - Jul 2010

Fingerprint

Omentum
Stromal Cells
Regeneration
Swine
Fats
Cardiac Myocytes
Endothelial Cells
Intra-Abdominal Fat
Angiogenesis Inducing Agents
Conditioned Culture Medium
Cell- and Tissue-Based Therapy
Morphogenesis
Adipocytes
Ligation
Blood Vessels
Monocytes
Histology
Cardiovascular Diseases
Arteries
Myocardial Infarction

Keywords

  • Fat
  • Heart infarct
  • Omentum
  • Stromal cells
  • Transplantation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Omentum-derived stromal cells improve myocardial regeneration in pig post-infarcted heart through a potent paracrine mechanism. / De Siena, Rocco; Balducci, Luigi; Blasi, Antonella; Montanaro, Manuela Gessica; Saldarelli, Marilisa; Saponaro, Vittorio; Martino, Carmela; Logrieco, Gaetano; Soleti, Antonio; Fiobellot, Simona; Madeddu, Paolo; Rossi, Giacomo; Ribatti, Domenico; Crovace, Antonio; Cristini, Silvia; Invernici, Gloria; Parati, Eugenio Agostino; Alessandri, Giulio.

In: Experimental Cell Research, Vol. 316, No. 11, 07.2010, p. 1804-1815.

Research output: Contribution to journalArticle

De Siena, R, Balducci, L, Blasi, A, Montanaro, MG, Saldarelli, M, Saponaro, V, Martino, C, Logrieco, G, Soleti, A, Fiobellot, S, Madeddu, P, Rossi, G, Ribatti, D, Crovace, A, Cristini, S, Invernici, G, Parati, EA & Alessandri, G 2010, 'Omentum-derived stromal cells improve myocardial regeneration in pig post-infarcted heart through a potent paracrine mechanism', Experimental Cell Research, vol. 316, no. 11, pp. 1804-1815. https://doi.org/10.1016/j.yexcr.2010.02.009
De Siena, Rocco ; Balducci, Luigi ; Blasi, Antonella ; Montanaro, Manuela Gessica ; Saldarelli, Marilisa ; Saponaro, Vittorio ; Martino, Carmela ; Logrieco, Gaetano ; Soleti, Antonio ; Fiobellot, Simona ; Madeddu, Paolo ; Rossi, Giacomo ; Ribatti, Domenico ; Crovace, Antonio ; Cristini, Silvia ; Invernici, Gloria ; Parati, Eugenio Agostino ; Alessandri, Giulio. / Omentum-derived stromal cells improve myocardial regeneration in pig post-infarcted heart through a potent paracrine mechanism. In: Experimental Cell Research. 2010 ; Vol. 316, No. 11. pp. 1804-1815.
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AU - Madeddu, Paolo

AU - Rossi, Giacomo

AU - Ribatti, Domenico

AU - Crovace, Antonio

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