Oncogenes belonging to the CSF-1 transduction pathway direct p53 tumor suppressor effects to monocytic differentiation in 32D cells

Roberta Martinelli, Giovanni Blandino, Raffaella Scardigli, Marco Crescenzi, Daniela Lombardi, Ada Sacchi, Silvia Soddu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Expression of exogenous wt-p53 in different tumor cell lines can induce growth arrest, apoptosis, or differentiation. Several experimental works have highlighted the relevance of cellular context in the determination of p53-mediated final outcomes. We recently observed that these diverse wt-p53 effects can also be induced by overexpressing wt-p53 in a single cell type - the 32D myeloid progenitors - transformed with different activated oncogenes. Here we show that 32D cells transformed with two different oncogenes, v-src or c-fms [S301,F969], both belonging to the CSF-1 transduction pathway, respond to exogenous wt-p53 expression with the same final outcome-monocytic differentiation. This result is particularly significant since 32D cells do not spontaneously express the CSF-1 receptor, whereas they undergo granulocytic differentiation upon G-CSF stimulation. These data strongly support the idea that wt-p53 suppressing effects result from interactions between p53 activity and the signaling pathways activated in different transformed cells.

Original languageEnglish
Pages (from-to)607-611
Number of pages5
JournalOncogene
Volume15
Issue number5
Publication statusPublished - 1997

Fingerprint

Macrophage Colony-Stimulating Factor
Oncogenes
Macrophage Colony-Stimulating Factor Receptors
src Genes
Myeloid Progenitor Cells
Neoplasms
Granulocyte Colony-Stimulating Factor
Tumor Cell Line
Apoptosis
Growth

Keywords

  • Cellular environment
  • Differentiation
  • Tumor suppression

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Oncogenes belonging to the CSF-1 transduction pathway direct p53 tumor suppressor effects to monocytic differentiation in 32D cells. / Martinelli, Roberta; Blandino, Giovanni; Scardigli, Raffaella; Crescenzi, Marco; Lombardi, Daniela; Sacchi, Ada; Soddu, Silvia.

In: Oncogene, Vol. 15, No. 5, 1997, p. 607-611.

Research output: Contribution to journalArticle

Martinelli, Roberta ; Blandino, Giovanni ; Scardigli, Raffaella ; Crescenzi, Marco ; Lombardi, Daniela ; Sacchi, Ada ; Soddu, Silvia. / Oncogenes belonging to the CSF-1 transduction pathway direct p53 tumor suppressor effects to monocytic differentiation in 32D cells. In: Oncogene. 1997 ; Vol. 15, No. 5. pp. 607-611.
@article{ab27774ab30f460b807a84171be28f96,
title = "Oncogenes belonging to the CSF-1 transduction pathway direct p53 tumor suppressor effects to monocytic differentiation in 32D cells",
abstract = "Expression of exogenous wt-p53 in different tumor cell lines can induce growth arrest, apoptosis, or differentiation. Several experimental works have highlighted the relevance of cellular context in the determination of p53-mediated final outcomes. We recently observed that these diverse wt-p53 effects can also be induced by overexpressing wt-p53 in a single cell type - the 32D myeloid progenitors - transformed with different activated oncogenes. Here we show that 32D cells transformed with two different oncogenes, v-src or c-fms [S301,F969], both belonging to the CSF-1 transduction pathway, respond to exogenous wt-p53 expression with the same final outcome-monocytic differentiation. This result is particularly significant since 32D cells do not spontaneously express the CSF-1 receptor, whereas they undergo granulocytic differentiation upon G-CSF stimulation. These data strongly support the idea that wt-p53 suppressing effects result from interactions between p53 activity and the signaling pathways activated in different transformed cells.",
keywords = "Cellular environment, Differentiation, Tumor suppression",
author = "Roberta Martinelli and Giovanni Blandino and Raffaella Scardigli and Marco Crescenzi and Daniela Lombardi and Ada Sacchi and Silvia Soddu",
year = "1997",
language = "English",
volume = "15",
pages = "607--611",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Oncogenes belonging to the CSF-1 transduction pathway direct p53 tumor suppressor effects to monocytic differentiation in 32D cells

AU - Martinelli, Roberta

AU - Blandino, Giovanni

AU - Scardigli, Raffaella

AU - Crescenzi, Marco

AU - Lombardi, Daniela

AU - Sacchi, Ada

AU - Soddu, Silvia

PY - 1997

Y1 - 1997

N2 - Expression of exogenous wt-p53 in different tumor cell lines can induce growth arrest, apoptosis, or differentiation. Several experimental works have highlighted the relevance of cellular context in the determination of p53-mediated final outcomes. We recently observed that these diverse wt-p53 effects can also be induced by overexpressing wt-p53 in a single cell type - the 32D myeloid progenitors - transformed with different activated oncogenes. Here we show that 32D cells transformed with two different oncogenes, v-src or c-fms [S301,F969], both belonging to the CSF-1 transduction pathway, respond to exogenous wt-p53 expression with the same final outcome-monocytic differentiation. This result is particularly significant since 32D cells do not spontaneously express the CSF-1 receptor, whereas they undergo granulocytic differentiation upon G-CSF stimulation. These data strongly support the idea that wt-p53 suppressing effects result from interactions between p53 activity and the signaling pathways activated in different transformed cells.

AB - Expression of exogenous wt-p53 in different tumor cell lines can induce growth arrest, apoptosis, or differentiation. Several experimental works have highlighted the relevance of cellular context in the determination of p53-mediated final outcomes. We recently observed that these diverse wt-p53 effects can also be induced by overexpressing wt-p53 in a single cell type - the 32D myeloid progenitors - transformed with different activated oncogenes. Here we show that 32D cells transformed with two different oncogenes, v-src or c-fms [S301,F969], both belonging to the CSF-1 transduction pathway, respond to exogenous wt-p53 expression with the same final outcome-monocytic differentiation. This result is particularly significant since 32D cells do not spontaneously express the CSF-1 receptor, whereas they undergo granulocytic differentiation upon G-CSF stimulation. These data strongly support the idea that wt-p53 suppressing effects result from interactions between p53 activity and the signaling pathways activated in different transformed cells.

KW - Cellular environment

KW - Differentiation

KW - Tumor suppression

UR - http://www.scopus.com/inward/record.url?scp=0030770916&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030770916&partnerID=8YFLogxK

M3 - Article

C2 - 9247315

AN - SCOPUS:0030770916

VL - 15

SP - 607

EP - 611

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 5

ER -