TY - JOUR
T1 - One-month persistence of inflammation and alteration of fibrotic marker and cytoskeletal proteins in rat kidney after Cd-doped silica nanoparticle instillation
AU - Coccini, Teresa
AU - Barni, Sergio
AU - Mustarelli, Piercarlo
AU - Locatelli, Carlo
AU - Roda, Elisa
PY - 2015/1/2
Y1 - 2015/1/2
N2 - In vivo effects of model cadmium-containing silica nanoparticles (SiNPs-Cd, 1mg/rat) were investigated by i.t. instillation in rats to evaluate potential effects on secondary target organ, e.g., kidney. Specific endpoints and pathological outcomes were focused to assess inflammation and fibrosis in renal tissue, 7 and 30 days after exposure to SiNPs-Cd, as well as to equivalent amount of CdCl2 or SiNPs. Immunohistochemistry was employed to investigate the presence/distribution of selected markers, i.e., (i) TGF-ß1, (ii) IL-6 (iii) collagen (type I), (iv) fibronectin, and (v) vimentin. An ongoing inflammatory process was demonstrated, together with a general overexpression of extracellular matrix components and alteration of cytoskeletal proteins, mainly in cortex and medulla, 7 days after SiNPs-Cd, lasting until 30th day.Less pronounced effects were observed after CdCl2, while SiNPs did not cause any insult except for IL-6 expression increase.Briefly, engineered SiNPs-Cd cause long-lasting injury in rat kidney, following a single pulmonary exposure. Renal response may be due to (i) translocation, i.e., nanoparticles migration from lung to systemic circulation, or (ii) secondary organ changes, caused by circulating inflammatory factors (e.g., IL-6, TGF-ß1) released from lung following local insult, or (iii) direct renal action of cadmium ions released from the absorbed SiNPs-Cd.
AB - In vivo effects of model cadmium-containing silica nanoparticles (SiNPs-Cd, 1mg/rat) were investigated by i.t. instillation in rats to evaluate potential effects on secondary target organ, e.g., kidney. Specific endpoints and pathological outcomes were focused to assess inflammation and fibrosis in renal tissue, 7 and 30 days after exposure to SiNPs-Cd, as well as to equivalent amount of CdCl2 or SiNPs. Immunohistochemistry was employed to investigate the presence/distribution of selected markers, i.e., (i) TGF-ß1, (ii) IL-6 (iii) collagen (type I), (iv) fibronectin, and (v) vimentin. An ongoing inflammatory process was demonstrated, together with a general overexpression of extracellular matrix components and alteration of cytoskeletal proteins, mainly in cortex and medulla, 7 days after SiNPs-Cd, lasting until 30th day.Less pronounced effects were observed after CdCl2, while SiNPs did not cause any insult except for IL-6 expression increase.Briefly, engineered SiNPs-Cd cause long-lasting injury in rat kidney, following a single pulmonary exposure. Renal response may be due to (i) translocation, i.e., nanoparticles migration from lung to systemic circulation, or (ii) secondary organ changes, caused by circulating inflammatory factors (e.g., IL-6, TGF-ß1) released from lung following local insult, or (iii) direct renal action of cadmium ions released from the absorbed SiNPs-Cd.
KW - Cadmium
KW - Immunohistochemistry
KW - In vivo
KW - Nanotoxicology
KW - Renal
KW - Silica
UR - http://www.scopus.com/inward/record.url?scp=84919820429&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84919820429&partnerID=8YFLogxK
U2 - 10.1016/j.toxlet.2014.11.021
DO - 10.1016/j.toxlet.2014.11.021
M3 - Article
C2 - 25445720
AN - SCOPUS:84919820429
VL - 232
SP - 449
EP - 457
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
IS - 2
ER -