One-step cartilage repair with minced chondral fragment onto a composite scaffold: An in vitro human study at low oxygen tension

A. G. Marmotti, G. M. Peretti, S. Mattia, L. Mangiavini, D. E. Bonasia, F. Dettoni, E. Bellato, S. Schwienbacher, F. Castoldi

Research output: Contribution to journalArticlepeer-review

Abstract

Minced cartilage fragments are a viable cell source for one stage cartilage repair. However, the joint surface is a low oxygen tension microenvironment and little evidence is present in literature regarding the behaviour of cartilage fragments in this peculiar condition. The aim of the study is i) to verify if low oxygen tension could negatively influence chondrocyte outgrowth from cartilage fragments into a Hyaluronic-Acid(HA)/fibrin scaffold and ii) to evaluate its effects on the behaviour of migrating chondrocyte, compared to normoxic condition. A slight decrease in chondrocyte migration and proliferation was observed in low oxygen tension cultures. Conversely, an increase in the expression of SOX9, β-catenin, HIFs, collagen-I and II (p<0.05) in migrating chondrocytes from low oxygen tension cultures was present. Thus, a long term- exposure at low oxygen tension seems to improve the chondrocytic phenotype expression of cell outgrowing from cartilage fragments onto a HA/fibrin scaffold.

Original languageEnglish
Pages (from-to)113-120
Number of pages8
JournalJournal of Biological Regulators and Homeostatic Agents
Volume31
Issue number4
Publication statusPublished - Sep 1 2017

Keywords

  • Composite scaffold
  • Low oxygen tension
  • Minced cartilage fragments
  • One-step cartilage repair

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Immunology and Allergy
  • Physiology
  • Immunology
  • Oncology
  • Endocrinology
  • Physiology (medical)
  • Cancer Research

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