One-step cloning of anti tissue transglutaminase scFv from subjects with celiac disease

Daniele Sblattero, Fiorella Florian, Elisabetta Azzoni, Fabiana Ziberna, Alberto Tommasini, Tarcisio Not, Alessandro Ventura, Andrew Bradbury, Roberto Marzari

Research output: Contribution to journalArticlepeer-review

Abstract

Celiac disease is characterized by intestinal mucosal injury and malabsorption precipitated by dietary exposure to gluten of some cereals with a prominent role being played by gliadins, specific antigenic determinants found in wheat gluten. Patients suffering from celiac disease have serum antibodies recognizing gliadin, as well as the endomysial autoantigen tissue transglutaminase. Phage display antibody libraries have revealed ectopic production of anti-transglutaminase antibodies by intestinal lymphocytes with a biased use of the VH5 antibody gene family. Here we report a study on the pairing of VH and VL families in the antibodies to transglutaminase. Our results led to the construction of small phage display antibody libraries based on the amplification of the two genes in the VH5 family from intestinal lymphocytes. This method can be used for the rapid characterization of the anti-transglutaminase response in a potentially large number of subjects including asymptomatic patients whose serum antibodies may be undetectable.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalJournal of Autoimmunity
Volume22
Issue number1
DOIs
Publication statusPublished - Feb 2004

Keywords

  • Antibody cloning
  • Autoimmunity
  • Gliadin
  • Phage display

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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