One-step osteochondral repair with cartilage fragments in a composite scaffold

A. Marmotti, M. Bruzzone, D. E. Bonasia, F. Castoldi, R. Rossi, L. Piras, A. Maiello, C. Realmuto, G. M. Peretti

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Purpose: This study proposes a single-step therapeutic approach for osteochondral defects using autologous cartilage fragments loaded onto a scaffold composed of a hyaluronic acid (HA) derivative, human fibrin glue (FG) and autologous platelet-rich-plasma (PRP), in a rabbit model. The aim is to demonstrate the in vitro outgrowth of chondrocytes from cartilage fragments and the in vivo formation of a functional repair tissue. Methods: In vitro: minced articular cartilage was loaded onto two different types of scaffold (paste or membrane) according to two different HA preparations (injectable HA-derivative or HA-derivative felt). In vivo: trochlear osteochondral defects were created in 50 adult rabbits, which were then assigned to 5 different treatment groups: cartilage fragments loaded onto membrane scaffolds with FG (Group 1) or without FG (Group 2); membrane scaffolds alone with FG (Group 3) or without FG (Group 4); empty defects (Group 5). Membrane scaffolds were used "in vivo" for simpler preparation and better adhesive properties. Repair processes were evaluated histologically and by immunohistochemistry at 1, 3, and 6 months. Results: An in vitro time-dependent cell outgrowth from cartilage fragments was observed with both types of scaffolds. At 6 months, in vivo, cartilage fragment-loaded scaffolds induced significantly better repair tissue than the scaffold alone using histological scoring. Repair in Group 2 was superior to that in any of the control groups (p <0.05). Conclusion: Autologous cartilage fragments loaded onto an HA felt/FG/PRP-scaffold provided an efficient cell source, and allowed for an improvement of the repair process of ostechondral defects in a rabbit model. Human FG, however, hampered the rabbit healing process. These results may have clinical relevance as they show the potential of a novel one-stage repair technique for osteochondral defects.

Original languageEnglish
Pages (from-to)2590-2601
Number of pages12
JournalKnee Surgery, Sports Traumatology, Arthroscopy
Volume20
Issue number12
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Fibrin Tissue Adhesive
Cartilage
Hyaluronic Acid
Rabbits
Platelet-Rich Plasma
Membranes
Tissue Scaffolds
Articular Cartilage
Chondrocytes
Ointments
Adhesives
Immunohistochemistry
Control Groups
Injections
Therapeutics
In Vitro Techniques

Keywords

  • Cartilage repair
  • Fibrin glue
  • Hyaluronic acid
  • Minced cartilage fragments
  • Platelet-rich-plasma
  • Scaffold

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

Cite this

One-step osteochondral repair with cartilage fragments in a composite scaffold. / Marmotti, A.; Bruzzone, M.; Bonasia, D. E.; Castoldi, F.; Rossi, R.; Piras, L.; Maiello, A.; Realmuto, C.; Peretti, G. M.

In: Knee Surgery, Sports Traumatology, Arthroscopy, Vol. 20, No. 12, 12.2012, p. 2590-2601.

Research output: Contribution to journalArticle

Marmotti, A, Bruzzone, M, Bonasia, DE, Castoldi, F, Rossi, R, Piras, L, Maiello, A, Realmuto, C & Peretti, GM 2012, 'One-step osteochondral repair with cartilage fragments in a composite scaffold', Knee Surgery, Sports Traumatology, Arthroscopy, vol. 20, no. 12, pp. 2590-2601. https://doi.org/10.1007/s00167-012-1920-y
Marmotti, A. ; Bruzzone, M. ; Bonasia, D. E. ; Castoldi, F. ; Rossi, R. ; Piras, L. ; Maiello, A. ; Realmuto, C. ; Peretti, G. M. / One-step osteochondral repair with cartilage fragments in a composite scaffold. In: Knee Surgery, Sports Traumatology, Arthroscopy. 2012 ; Vol. 20, No. 12. pp. 2590-2601.
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AU - Bonasia, D. E.

AU - Castoldi, F.

AU - Rossi, R.

AU - Piras, L.

AU - Maiello, A.

AU - Realmuto, C.

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