On/off TLR signaling decides proinflammatory or tolerogenic dendritic cell maturation upon CD1D-mediated interaction with invariant NKT cells

Simone Caielli, Cristina Conforti-Andreoni, Caterina Di Pietro, Vera Usuelli, Ester Badami, Maria Luisa Malosio, Marika Falcone

Research output: Contribution to journalArticle

Abstract

Invariant NKT (iNKT) cells play an effector/adjuvant function during antimicrobial and antitumoral immunity and a regulatory role to induce immune tolerance and prevent autoimmunity. iNKT cells that differentially modulate adaptive immunity do not bear a unique phenotype and/or specific cytokine secretion profile, thus opening questions on how a single T cell subset can exert opposite immunological tasks. In this study, we show that iNKT cells perform their dual roles througha single mechanism of action relying on the cognate interaction with myeloid dendritic cells (DCs) and leading to opposite effects depending on the presence of other maturation stimuli simultaneously acting on DCs. The contact of murine purified iNKT cells with immature autologous DCs directly triggers the tolerogenic maturation of DCs, rendering them able to induce regulatory T cell differentiation and prevent autoimmune diabetes in vivo. Conversely, the interaction of the same purified iNKT cells with DCs, in the presence of simultaneous TLR4 stimulation, significantly enhances proinflammatory DC maturation and IL-12 secretion. The different iNKT cell effects are mediated through distinct mechanisms and activation of different molecular pathways within the DC: CD1d signaling and activation of the ERK1/2 pathway for the tolerogenic action, and CD40-CD40L interaction and NF-κB activation for the adjuvant effect. Our data suggest that the DC decision to undergo proinflammatory or tolerogenic maturation results from the integration of different signals received at the time of iNKT cell contact and could have important therapeutic implications for exploiting iNKT cell adjuvant/regulatory properties in autoimmune diseases, infections, and cancer.

Original languageEnglish
Pages (from-to)7317-7329
Number of pages13
JournalJournal of Immunology
Volume185
Issue number12
DOIs
Publication statusPublished - Dec 15 2010

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Natural Killer T-Cells
Dendritic Cells
Immune Tolerance
CD40 Ligand
MAP Kinase Signaling System
T-Lymphocyte Subsets
Adaptive Immunity
Regulatory T-Lymphocytes
Myeloid Cells
Interleukin-12
Autoimmunity
Type 1 Diabetes Mellitus
Autoimmune Diseases
Cell Differentiation
Immunity
Cytokines
Phenotype
Infection

ASJC Scopus subject areas

  • Immunology

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On/off TLR signaling decides proinflammatory or tolerogenic dendritic cell maturation upon CD1D-mediated interaction with invariant NKT cells. / Caielli, Simone; Conforti-Andreoni, Cristina; Di Pietro, Caterina; Usuelli, Vera; Badami, Ester; Malosio, Maria Luisa; Falcone, Marika.

In: Journal of Immunology, Vol. 185, No. 12, 15.12.2010, p. 7317-7329.

Research output: Contribution to journalArticle

Caielli, Simone ; Conforti-Andreoni, Cristina ; Di Pietro, Caterina ; Usuelli, Vera ; Badami, Ester ; Malosio, Maria Luisa ; Falcone, Marika. / On/off TLR signaling decides proinflammatory or tolerogenic dendritic cell maturation upon CD1D-mediated interaction with invariant NKT cells. In: Journal of Immunology. 2010 ; Vol. 185, No. 12. pp. 7317-7329.
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