ONYX-015 Enhances Radiation-Induced Death of Human Anaplastic Thyroid Carcinoma Cells

Giuseppe Portella, Roberto Pacelli, Silvana Libertini, Laura Cella, Giancarlo Vecchio, Marco Salvatore, Alfredo Fusco

Research output: Contribution to journalArticlepeer-review

Abstract

ONYX-015 is a genetically modified adenovirus with a deletion of the E1B early gene and therefore is designed to replicate preferentially in p53-mutated cells causing their death. We previously demonstrated that the ONYX-015 virus kills anaplastic thyroid carcinoma (ATC) cells and enhances the anti-neoplastic effects of doxorubicin and paclitaxel. Here we report that ONYX-015 increased the cytopathic effect of radiotherapy in three ATC cell lines. In fact, ONYX-015 and radiation induced a significant cytopathic effect on ATC cells. DNA fragmentation analysis showed that ATC ONYX-015-treated cells were very sensitive to radiation-induced apoptosis. In addition, low doses of ONYX-015 associated with a single radiation dose of 10 Gy delayed the growth of a xenograft tumor induced by ARO cells in athymic mice. Our results suggest that the combination of ONYX-015 and radiotherapy should be considered for experimental trials in patients with anaplastic thyroid carcinoma.

Original languageEnglish
Pages (from-to)5027-5032
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume88
Issue number10
DOIs
Publication statusPublished - Oct 2003

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

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