OPA1 mutations associated with dominant optic atrophy impair oxidative phosphorylation and mitochondrial fusion

Claudia Zanna, Anna Ghelli, Anna Maria Porcelli, Mariusz Karbowski, Richard J. Youle, Simone Schimpf, Bernd Wissinger, Marcello Pinti, Andrea Cossarizza, Sara Vidoni, Maria Lucia Valentino, Michela Rugolo, Valerio Carelli

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

Dominant optic atrophy (DOA) is characterized by retinal ganglion cell degeneration leading to optic neuropathy. A subset of DOA is caused by mutations in the OPA1 gene, encoding for a dynamin-related GTPase required for mitochondrial fusion. The functional consequences of OPA1 mutations in DOA patients are still poorly understood. This study investigated the effect of five different OPA1 pathogenic mutations on the energetic efficiency and mitochondrial network dynamics of skin fibroblasts from patients. Although DOA fibroblasts maintained their ATP levels and grew in galactose medium, i.e. under forced oxidative metabolism, a significant impairment in mitochondrial ATP synthesis driven by complex I substrates was found. Furthermore, balloon-like structures in the mitochondrial reticulum were observed in galactose medium and mitochondrial fusion was completely inhibited in about 50% of DOA fibroblasts, but not in control cells. Respiratory complex assembly and the expression level of complex I subunits were similar in control and DOA fibroblasts. Co-immunoprecipitation experiments revealed that OPA1 directly interacts with subunits of complexes I, II and III, but not IV and with apoptosis inducing factor. The results disclose a novel link between OPA1, apoptosis inducing factor and the respiratory complexes that may shed some light on the pathogenic mechanism of DOA.

Original languageEnglish
Pages (from-to)352-367
Number of pages16
JournalBrain
Volume131
Issue number2
DOIs
Publication statusPublished - Feb 2008

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Autosomal Dominant Optic Atrophy
Mitochondrial Dynamics
Oxidative Phosphorylation
Mutation
Fibroblasts
Apoptosis Inducing Factor
Galactose
Adenosine Triphosphate
Dynamins
Reticulum
Optic Nerve Diseases
Retinal Ganglion Cells
GTP Phosphohydrolases
Immunoprecipitation
Skin

Keywords

  • AIF
  • DOA
  • Mitochondrial fusion
  • OPA1
  • Oxidative phosphorylation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

OPA1 mutations associated with dominant optic atrophy impair oxidative phosphorylation and mitochondrial fusion. / Zanna, Claudia; Ghelli, Anna; Porcelli, Anna Maria; Karbowski, Mariusz; Youle, Richard J.; Schimpf, Simone; Wissinger, Bernd; Pinti, Marcello; Cossarizza, Andrea; Vidoni, Sara; Valentino, Maria Lucia; Rugolo, Michela; Carelli, Valerio.

In: Brain, Vol. 131, No. 2, 02.2008, p. 352-367.

Research output: Contribution to journalArticle

Zanna, C, Ghelli, A, Porcelli, AM, Karbowski, M, Youle, RJ, Schimpf, S, Wissinger, B, Pinti, M, Cossarizza, A, Vidoni, S, Valentino, ML, Rugolo, M & Carelli, V 2008, 'OPA1 mutations associated with dominant optic atrophy impair oxidative phosphorylation and mitochondrial fusion', Brain, vol. 131, no. 2, pp. 352-367. https://doi.org/10.1093/brain/awm335
Zanna, Claudia ; Ghelli, Anna ; Porcelli, Anna Maria ; Karbowski, Mariusz ; Youle, Richard J. ; Schimpf, Simone ; Wissinger, Bernd ; Pinti, Marcello ; Cossarizza, Andrea ; Vidoni, Sara ; Valentino, Maria Lucia ; Rugolo, Michela ; Carelli, Valerio. / OPA1 mutations associated with dominant optic atrophy impair oxidative phosphorylation and mitochondrial fusion. In: Brain. 2008 ; Vol. 131, No. 2. pp. 352-367.
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