Open-chain peptides obtained by acidic hydrolytic cleavage of cyclosporin A

F. Magni, C. Arcelloni, R. Paroni, I. Fermo, P. A. Bonini, M. Del Puppo, A. Manzocchi, M. Galli Kienle

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Hydrolysis of cyclosporin A (CsA) was studied in order to clarify the still undefined point of attack of the acidic degradation. Among ether extractable and water-soluble products formed from CsA in HCl, two open-chain peptides were isolated by high-performance liquid chromatography which were identified as the deca- and nonapeptides deriving from CsA through the hydrolytic cleavage of amino acid residue 11 and both residues 11 and 10, respectively. Identification was carried out by fast atom bombardment tandem mass spectrometry.

Original languageEnglish
Pages (from-to)514-518
Number of pages5
JournalBiological Mass Spectrometry
Volume23
Issue number8
DOIs
Publication statusPublished - Aug 1994

Fingerprint

Cyclosporine
Peptides
Fast Atom Bombardment Mass Spectrometry
High performance liquid chromatography
Tandem Mass Spectrometry
Ether
Mass spectrometry
Hydrolysis
High Pressure Liquid Chromatography
Amino Acids
Degradation
Atoms
Water

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

Cite this

Magni, F., Arcelloni, C., Paroni, R., Fermo, I., Bonini, P. A., Del Puppo, M., ... Galli Kienle, M. (1994). Open-chain peptides obtained by acidic hydrolytic cleavage of cyclosporin A. Biological Mass Spectrometry, 23(8), 514-518. https://doi.org/10.1002/bms.1200230809

Open-chain peptides obtained by acidic hydrolytic cleavage of cyclosporin A. / Magni, F.; Arcelloni, C.; Paroni, R.; Fermo, I.; Bonini, P. A.; Del Puppo, M.; Manzocchi, A.; Galli Kienle, M.

In: Biological Mass Spectrometry, Vol. 23, No. 8, 08.1994, p. 514-518.

Research output: Contribution to journalArticle

Magni, F, Arcelloni, C, Paroni, R, Fermo, I, Bonini, PA, Del Puppo, M, Manzocchi, A & Galli Kienle, M 1994, 'Open-chain peptides obtained by acidic hydrolytic cleavage of cyclosporin A', Biological Mass Spectrometry, vol. 23, no. 8, pp. 514-518. https://doi.org/10.1002/bms.1200230809
Magni, F. ; Arcelloni, C. ; Paroni, R. ; Fermo, I. ; Bonini, P. A. ; Del Puppo, M. ; Manzocchi, A. ; Galli Kienle, M. / Open-chain peptides obtained by acidic hydrolytic cleavage of cyclosporin A. In: Biological Mass Spectrometry. 1994 ; Vol. 23, No. 8. pp. 514-518.
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