Abstract
Although the distribution of opioid receptors is central to the patch-matrix model of neostriatal organization, it has been unclear whether these receptors are located post-synaptically. Moreover, it has not yet been clarified whether opioid receptor activation in neostriatum results in the modulation of calcium and/or potassium conductances. To test this, neostriatal neurons were acutely isolated and their sensitivity to opioid receptor agonists determined. At nanomolar. At nanomolar concentrations, both the μ-agonist [d-Ala2, MePhe4, Gly-ol5]-enkephalin (DAMGO) and the δ-agonist [d-Pen2, d-Pen5]-enkephalin (DPEPE) reversibly decreased whole-cell calcium currents in medium-sized neurons. These effects were blocked by the opiate antagonist naloxone. These findings argue that activation of post-synaptic, opioid receptors is capable of modulating the excitability of neostriatal neurons.
| Original language | English |
|---|---|
| Pages (from-to) | 339-343 |
| Number of pages | 5 |
| Journal | Brain Research |
| Volume | 642 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Apr 11 1994 |
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Keywords
- Basal ganglia
- High-voltage activated (HVA) current
- Opioid peptide
- Whole-cell patch clamp
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Opioids decrease high-voltage activated calcium currents in acutely dissociated neostriatal neurons. / Stefani, A.; Surmeier, D. J.; Bernardi, G.
In: Brain Research, Vol. 642, No. 1-2, 11.04.1994, p. 339-343.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Opioids decrease high-voltage activated calcium currents in acutely dissociated neostriatal neurons
AU - Stefani, A.
AU - Surmeier, D. J.
AU - Bernardi, G.
PY - 1994/4/11
Y1 - 1994/4/11
N2 - Although the distribution of opioid receptors is central to the patch-matrix model of neostriatal organization, it has been unclear whether these receptors are located post-synaptically. Moreover, it has not yet been clarified whether opioid receptor activation in neostriatum results in the modulation of calcium and/or potassium conductances. To test this, neostriatal neurons were acutely isolated and their sensitivity to opioid receptor agonists determined. At nanomolar. At nanomolar concentrations, both the μ-agonist [d-Ala2, MePhe4, Gly-ol5]-enkephalin (DAMGO) and the δ-agonist [d-Pen2, d-Pen5]-enkephalin (DPEPE) reversibly decreased whole-cell calcium currents in medium-sized neurons. These effects were blocked by the opiate antagonist naloxone. These findings argue that activation of post-synaptic, opioid receptors is capable of modulating the excitability of neostriatal neurons.
AB - Although the distribution of opioid receptors is central to the patch-matrix model of neostriatal organization, it has been unclear whether these receptors are located post-synaptically. Moreover, it has not yet been clarified whether opioid receptor activation in neostriatum results in the modulation of calcium and/or potassium conductances. To test this, neostriatal neurons were acutely isolated and their sensitivity to opioid receptor agonists determined. At nanomolar. At nanomolar concentrations, both the μ-agonist [d-Ala2, MePhe4, Gly-ol5]-enkephalin (DAMGO) and the δ-agonist [d-Pen2, d-Pen5]-enkephalin (DPEPE) reversibly decreased whole-cell calcium currents in medium-sized neurons. These effects were blocked by the opiate antagonist naloxone. These findings argue that activation of post-synaptic, opioid receptors is capable of modulating the excitability of neostriatal neurons.
KW - Basal ganglia
KW - High-voltage activated (HVA) current
KW - Opioid peptide
KW - Whole-cell patch clamp
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U2 - 10.1016/0006-8993(94)90940-7
DO - 10.1016/0006-8993(94)90940-7
M3 - Article
C2 - 8032898
AN - SCOPUS:0028220070
VL - 642
SP - 339
EP - 343
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -