Opposing regulation of amyloid precursor protein by ionotropic and metabotropic glutamate receptors

Alessandra Valerio, Antonella Alberici, Marta Paterlini, Mariagrazia Grilli, Paola Galli, Marina Pizzi, Maurizio Memo, Pierfranco Spano

Research output: Contribution to journalArticle

Abstract

The effects of the ionotropic glutamate receptor (iGluR) selective agonist N-methyl-d-aspartate (NMDA) on amyloid precursor protein (APP) levels were investigated in primary cultures of rat cerebellar granule cells. Both immunocytochemistry and immunoblotting techniques showed increased APP levels 4 h after a 15 min pulse with NMDA. This effect was completely prevented by incubating the neurones in the presence of the selective metabotropic GluR (mGluR) agonist 1S,3R-ACPD. This phenomenon was related, in terms of doses and time, with the observed 1S,3R-ACPD-mediated protection on NMDA-induced granule cell death. Our findings indicate that APP metabolism is differentially regulated by the stimulation of various GluR subtypes. The GluRmediated changes in APP content might participate in the control of neuronal viability.

Original languageEnglish
Pages (from-to)1317-1321
Number of pages5
JournalNeuroReport
Volume6
Issue number9
Publication statusPublished - 1995

Keywords

  • Amyloid precursor protein
  • Cerebellar granule cells
  • Glutamate receptors

ASJC Scopus subject areas

  • Neuroscience(all)

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  • Cite this

    Valerio, A., Alberici, A., Paterlini, M., Grilli, M., Galli, P., Pizzi, M., Memo, M., & Spano, P. (1995). Opposing regulation of amyloid precursor protein by ionotropic and metabotropic glutamate receptors. NeuroReport, 6(9), 1317-1321.