Opposing regulation of tau protein levels by ionotropic and metabotropic glutamate receptors in human NT2 neurons

Marta Paterlini, Alessandra Valerio, Francesca Baruzzi, Maurizio Memo, Pierfranco Spano

Research output: Contribution to journalArticle

Abstract

Human NT2-N neurons derived from retinoic acid treatment of the NTera 2 cell line were used to determine the consequences of ionotropic glutamate receptor (iGluR) hyperstimulation and possible modulatory role(s) exerted by metabotropic glutamate receptor (mGluR) activation. We found that NT2-N neurons express the NR1 subunit of N-methyl-D-aspartate (NMDA) iGluRs and mRNA encoding the 1a isoform of mGluRs. A 15 min pulse with 100 μM NMDA induced an increase in the levels of tau proteins in NT2-N cells. This effect was prevented by incubating NT2-N neurons in the presence of the mGluR agonist (1S,3R)-1 aminocyclopentane-1,3-dicarboxylic acid (1S,3R-ACPD). This phenomenon was related, in terms of doses and time, with the observed 1S,3R- ACPD-mediated protection against NMDA-induced NT2-N cell death. Our findings suggest that iGluRs and mGluRs might participate in the control of human neuron viability by differentially affecting the expression of tau proteins.

Original languageEnglish
Pages (from-to)77-80
Number of pages4
JournalNeuroscience Letters
Volume243
Issue number1-3
DOIs
Publication statusPublished - Feb 27 1998

Fingerprint

Ionotropic Glutamate Receptors
tau Proteins
Metabotropic Glutamate Receptors
N-Methylaspartate
Neurons
Excitatory Amino Acid Agonists
Tretinoin
Protein Isoforms
Cell Death
Cell Line
Messenger RNA
1-amino-1,3-dicarboxycyclopentane

Keywords

  • Glutamate receptors
  • Human
  • Neurodegeneration
  • NT2-N neurons
  • Tau protein

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Opposing regulation of tau protein levels by ionotropic and metabotropic glutamate receptors in human NT2 neurons. / Paterlini, Marta; Valerio, Alessandra; Baruzzi, Francesca; Memo, Maurizio; Spano, Pierfranco.

In: Neuroscience Letters, Vol. 243, No. 1-3, 27.02.1998, p. 77-80.

Research output: Contribution to journalArticle

Paterlini, Marta ; Valerio, Alessandra ; Baruzzi, Francesca ; Memo, Maurizio ; Spano, Pierfranco. / Opposing regulation of tau protein levels by ionotropic and metabotropic glutamate receptors in human NT2 neurons. In: Neuroscience Letters. 1998 ; Vol. 243, No. 1-3. pp. 77-80.
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