Opposite effect of tumor necrosis factor α on granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor-dependent growth of normal and leukemic hemopoietic progenitors

W. Piacibello, F. Sanavio, A. Severino, S. Morelli, A. M. Vaira, A. Stacchini, M. Aglietta

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The effect of recombinant human tumor necrosis factor α (TNF-α) on normal and chronic myeloid leukemia granulocyte-macrophage progenitors (CFU-GM) growing in semisolid agar cultures in the presence of recombinant granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor was studied. Granulocyte-macrophage colony-stimulating factor-dependent growth of normal and chronic myeloid leukemia bone marrow CFU-GM was greatly enhanced by TNF-α at doses of 0.1 to 100 units/ml. Growth enhancement included neutrophil, eosinophil, and monocyte-macrophage colonies and clusters at 7 and 14 days of culture. Since similar results were achieved with highly enriched progenitor cell populations, devoid of accessory cells, an indirect effect on CFU-GM growth through the release by accessory cells of other cytokines upon TNF-α stimulation was thus ruled out. By contrast, the same doses of TNF-α inhibited the growth of normal CFU-GM in granulocyte colony-stimulating factor-dependent cultures. Taken together, our findings indicate that the final effect of TNF-α on normal bone marrow granulocyte-macrophage progenitor growth is dependent on the specific growth factor interacting with it, and that both normal and chronic myeloid leukemia CFU-GM are equally responsive to the combined effects of TNF-α and a given colony-stimulating factor.

Original languageEnglish
Pages (from-to)5065-5071
Number of pages7
JournalCancer Research
Volume50
Issue number16
Publication statusPublished - 1990

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Granulocyte-Macrophage Progenitor Cells
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Tumor Necrosis Factor-alpha
Growth
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Bone Marrow
Colony-Stimulating Factors
Eosinophils
Agar
Monocytes
Intercellular Signaling Peptides and Proteins
Neutrophils
Stem Cells
Macrophages
Cytokines
Population

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Opposite effect of tumor necrosis factor α on granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor-dependent growth of normal and leukemic hemopoietic progenitors. / Piacibello, W.; Sanavio, F.; Severino, A.; Morelli, S.; Vaira, A. M.; Stacchini, A.; Aglietta, M.

In: Cancer Research, Vol. 50, No. 16, 1990, p. 5065-5071.

Research output: Contribution to journalArticle

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abstract = "The effect of recombinant human tumor necrosis factor α (TNF-α) on normal and chronic myeloid leukemia granulocyte-macrophage progenitors (CFU-GM) growing in semisolid agar cultures in the presence of recombinant granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor was studied. Granulocyte-macrophage colony-stimulating factor-dependent growth of normal and chronic myeloid leukemia bone marrow CFU-GM was greatly enhanced by TNF-α at doses of 0.1 to 100 units/ml. Growth enhancement included neutrophil, eosinophil, and monocyte-macrophage colonies and clusters at 7 and 14 days of culture. Since similar results were achieved with highly enriched progenitor cell populations, devoid of accessory cells, an indirect effect on CFU-GM growth through the release by accessory cells of other cytokines upon TNF-α stimulation was thus ruled out. By contrast, the same doses of TNF-α inhibited the growth of normal CFU-GM in granulocyte colony-stimulating factor-dependent cultures. Taken together, our findings indicate that the final effect of TNF-α on normal bone marrow granulocyte-macrophage progenitor growth is dependent on the specific growth factor interacting with it, and that both normal and chronic myeloid leukemia CFU-GM are equally responsive to the combined effects of TNF-α and a given colony-stimulating factor.",
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T1 - Opposite effect of tumor necrosis factor α on granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor-dependent growth of normal and leukemic hemopoietic progenitors

AU - Piacibello, W.

AU - Sanavio, F.

AU - Severino, A.

AU - Morelli, S.

AU - Vaira, A. M.

AU - Stacchini, A.

AU - Aglietta, M.

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