Using a spectrophotometer with an internal integrating sphere, the absorption (μa) and reduced scattering (μs′) coefficients of in vitro epidermis were evaluated from reflectance and transmittance measurements. μa and μs′ varied from 24 to 0.2 cm-1 and from 32 to 21 cm-1 respectively, on passing from 400 to 800 nm. Moreover, using an external integrating sphere, the reflectance spectrum of in vivo skin was compared with the reflectance spectrum calculated with a Monte Carlo model, in which the mean values of μa and μs′ and different anisotropy parameters were used as input data. In vivo results show that the principle of similarity is entirely valid for wavelengths greater than 600 nm and may be considered a good approximation in the 400-600 nm band, and suggest that optical characteristics of in vivo skin may be inferred from reflectance measurements.
|Number of pages||14|
|Journal||Journal of Photochemistry and Photobiology B: Biology|
|Publication status||Published - Oct 30 1992|
- absorption coefficient
- Monte Carlo model.
- scattering coefficient
ASJC Scopus subject areas
- Plant Science
- Physical and Theoretical Chemistry