Optical recording of electrical activity in intact neuronal networks with random access second-harmonic generation microscopy

Leonardo Sacconi, Jonathan Mapelli, Daniela Gandolfi, Jacopo Lotti, Rodney P. O'Connor, Egidio D'Angelo, Francesco S. Pavone

Research output: Contribution to journalArticle

Abstract

One of the main challenges in understanding the central nervous system is to measure the network dynamics of neuronal assemblies, while preserving the computational role of individual neurons. However, this is not possible with current techniques. In this work, we combined the advantages of second-harmonic generation (SHG) with a random access (RA) excitation scheme to realize a new microscope (RASH) capable of optically recording fast membrane potential events occurring in a wide-field of view. The RASH microscope, in combination with bulk loading of tissue with FM4-64 dye, was used to simultaneously record electrical activity from clusters of Purkinje cells in acute cerebellar slices. Complex spikes, both synchronous and asynchronous, were optically recorded simultaneously across a given population of neurons. Spontaneous electrical activity was also monitored simultaneously in pairs of neurons, where action potentials were recorded without averaging across trials. These results show the strength of this technique in describing the temporal dynamics of neuronal assemblies, opening promising perspectives in understanding the computations of neuronal networks.

Original languageEnglish
Pages (from-to)14910-14921
Number of pages12
JournalOptics Express
Volume16
Issue number19
DOIs
Publication statusPublished - Sep 15 2008

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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