Abstract
Flow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows identification of apoptotic cells as a 'subdiploid' peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually to cell disruption. Thus, a major problem for the flow cytometric analysis of apoptotic populations is discrimination between debris and apoptotic cells. Here we demonstrate that the best parameter on which to make such a distinction is the DNA content, no matter what type of cell is studied. In contrast, discrimination between apoptotic, non-apoptotic cells, and debris is possible on the basis of scattering signals only in few selected cases, depending on the morphology of the intact cells.
| Original language | English |
|---|---|
| Pages (from-to) | 891-897 |
| Number of pages | 7 |
| Journal | Cytometry |
| Volume | 14 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 1993 |
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Keywords
- Apoptosis
- Cell morphology
- Electron microscopy
- Flow cytometry
ASJC Scopus subject areas
- Hematology
- Cell Biology
- Pathology and Forensic Medicine
- Biophysics
- Endocrinology
Cite this
Optimal detection of apoptosis by flow cytometry depends on cell morphology. / Zamai, L.; Falcieri, E.; Zauli, G.; Cataldi, A.; Vitale, M.
In: Cytometry, Vol. 14, No. 8, 1993, p. 891-897.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Optimal detection of apoptosis by flow cytometry depends on cell morphology
AU - Zamai, L.
AU - Falcieri, E.
AU - Zauli, G.
AU - Cataldi, A.
AU - Vitale, M.
PY - 1993
Y1 - 1993
N2 - Flow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows identification of apoptotic cells as a 'subdiploid' peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually to cell disruption. Thus, a major problem for the flow cytometric analysis of apoptotic populations is discrimination between debris and apoptotic cells. Here we demonstrate that the best parameter on which to make such a distinction is the DNA content, no matter what type of cell is studied. In contrast, discrimination between apoptotic, non-apoptotic cells, and debris is possible on the basis of scattering signals only in few selected cases, depending on the morphology of the intact cells.
AB - Flow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows identification of apoptotic cells as a 'subdiploid' peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually to cell disruption. Thus, a major problem for the flow cytometric analysis of apoptotic populations is discrimination between debris and apoptotic cells. Here we demonstrate that the best parameter on which to make such a distinction is the DNA content, no matter what type of cell is studied. In contrast, discrimination between apoptotic, non-apoptotic cells, and debris is possible on the basis of scattering signals only in few selected cases, depending on the morphology of the intact cells.
KW - Apoptosis
KW - Cell morphology
KW - Electron microscopy
KW - Flow cytometry
UR - http://www.scopus.com/inward/record.url?scp=0027483849&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027483849&partnerID=8YFLogxK
U2 - 10.1002/cyto.990140807
DO - 10.1002/cyto.990140807
M3 - Article
VL - 14
SP - 891
EP - 897
JO - Cytometry Part B - Clinical Cytometry
JF - Cytometry Part B - Clinical Cytometry
SN - 1552-4949
IS - 8
ER -