Optimal detection of apoptosis by flow cytometry depends on cell morphology

L. Zamai, E. Falcieri, G. Zauli, A. Cataldi, M. Vitale

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Flow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows identification of apoptotic cells as a 'subdiploid' peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually to cell disruption. Thus, a major problem for the flow cytometric analysis of apoptotic populations is discrimination between debris and apoptotic cells. Here we demonstrate that the best parameter on which to make such a distinction is the DNA content, no matter what type of cell is studied. In contrast, discrimination between apoptotic, non-apoptotic cells, and debris is possible on the basis of scattering signals only in few selected cases, depending on the morphology of the intact cells.

Original languageEnglish
Pages (from-to)891-897
Number of pages7
JournalCytometry
Volume14
Issue number8
DOIs
Publication statusPublished - 1993

Fingerprint

Flow Cytometry
Apoptosis
DNA
Chromatin
Population

Keywords

  • Apoptosis
  • Cell morphology
  • Electron microscopy
  • Flow cytometry

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

Zamai, L., Falcieri, E., Zauli, G., Cataldi, A., & Vitale, M. (1993). Optimal detection of apoptosis by flow cytometry depends on cell morphology. Cytometry, 14(8), 891-897. https://doi.org/10.1002/cyto.990140807

Optimal detection of apoptosis by flow cytometry depends on cell morphology. / Zamai, L.; Falcieri, E.; Zauli, G.; Cataldi, A.; Vitale, M.

In: Cytometry, Vol. 14, No. 8, 1993, p. 891-897.

Research output: Contribution to journalArticle

Zamai, L, Falcieri, E, Zauli, G, Cataldi, A & Vitale, M 1993, 'Optimal detection of apoptosis by flow cytometry depends on cell morphology', Cytometry, vol. 14, no. 8, pp. 891-897. https://doi.org/10.1002/cyto.990140807
Zamai, L. ; Falcieri, E. ; Zauli, G. ; Cataldi, A. ; Vitale, M. / Optimal detection of apoptosis by flow cytometry depends on cell morphology. In: Cytometry. 1993 ; Vol. 14, No. 8. pp. 891-897.
@article{6fd2e20bcb4a4a0285637d9ab733a03c,
title = "Optimal detection of apoptosis by flow cytometry depends on cell morphology",
abstract = "Flow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows identification of apoptotic cells as a 'subdiploid' peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually to cell disruption. Thus, a major problem for the flow cytometric analysis of apoptotic populations is discrimination between debris and apoptotic cells. Here we demonstrate that the best parameter on which to make such a distinction is the DNA content, no matter what type of cell is studied. In contrast, discrimination between apoptotic, non-apoptotic cells, and debris is possible on the basis of scattering signals only in few selected cases, depending on the morphology of the intact cells.",
keywords = "Apoptosis, Cell morphology, Electron microscopy, Flow cytometry",
author = "L. Zamai and E. Falcieri and G. Zauli and A. Cataldi and M. Vitale",
year = "1993",
doi = "10.1002/cyto.990140807",
language = "English",
volume = "14",
pages = "891--897",
journal = "Cytometry Part B - Clinical Cytometry",
issn = "1552-4949",
publisher = "Wiley-Liss Inc.",
number = "8",

}

TY - JOUR

T1 - Optimal detection of apoptosis by flow cytometry depends on cell morphology

AU - Zamai, L.

AU - Falcieri, E.

AU - Zauli, G.

AU - Cataldi, A.

AU - Vitale, M.

PY - 1993

Y1 - 1993

N2 - Flow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows identification of apoptotic cells as a 'subdiploid' peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually to cell disruption. Thus, a major problem for the flow cytometric analysis of apoptotic populations is discrimination between debris and apoptotic cells. Here we demonstrate that the best parameter on which to make such a distinction is the DNA content, no matter what type of cell is studied. In contrast, discrimination between apoptotic, non-apoptotic cells, and debris is possible on the basis of scattering signals only in few selected cases, depending on the morphology of the intact cells.

AB - Flow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows identification of apoptotic cells as a 'subdiploid' peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually to cell disruption. Thus, a major problem for the flow cytometric analysis of apoptotic populations is discrimination between debris and apoptotic cells. Here we demonstrate that the best parameter on which to make such a distinction is the DNA content, no matter what type of cell is studied. In contrast, discrimination between apoptotic, non-apoptotic cells, and debris is possible on the basis of scattering signals only in few selected cases, depending on the morphology of the intact cells.

KW - Apoptosis

KW - Cell morphology

KW - Electron microscopy

KW - Flow cytometry

UR - http://www.scopus.com/inward/record.url?scp=0027483849&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027483849&partnerID=8YFLogxK

U2 - 10.1002/cyto.990140807

DO - 10.1002/cyto.990140807

M3 - Article

VL - 14

SP - 891

EP - 897

JO - Cytometry Part B - Clinical Cytometry

JF - Cytometry Part B - Clinical Cytometry

SN - 1552-4949

IS - 8

ER -