Optimisation of electrotransfer of plasmid into skeletal muscle by pretreatment with hyaluronidase - Increased expression with reduced muscle damage

J. M. McMahon, E. Signori, K. E. Wells, V. M. Fazio, D. J. Wells

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

The efficiency of plasmid gene transfer to skeletal muscle can be significantly improved by the application of an electrical field to the muscle following injection of plasmid DNA. However, this electrotransfer is associated with significant muscle damage which may result in substantial loss of transfected muscle fibres. Reduction of the voltage used in the technique can result in a decrease in muscle damage, with a concomitant reduction in expression, but without a significant decrease in the number of transfected fibres. Pre-treatment of the muscle with a solution of bovine hyaluronidase greatly increases the efficiency of plasmid gene transfer when used in conjunction with electrotransfer, but not when used alone. This combination treatment results in greatly enhanced levels of transfected muscle fibres without the increases in muscle damage associated with the electrotransfer process.

Original languageEnglish
Pages (from-to)1264-1270
Number of pages7
JournalGene Therapy
Volume8
Issue number16
DOIs
Publication statusPublished - 2001

Fingerprint

Hyaluronoglucosaminidase
Skeletal Muscle
Plasmids
Muscles
Genes
Injections
DNA
Therapeutics

Keywords

  • Electroporation
  • Fibres
  • Gene therapy
  • Muscular dystrophy
  • Plasmid
  • Skeletal muscle

ASJC Scopus subject areas

  • Genetics

Cite this

Optimisation of electrotransfer of plasmid into skeletal muscle by pretreatment with hyaluronidase - Increased expression with reduced muscle damage. / McMahon, J. M.; Signori, E.; Wells, K. E.; Fazio, V. M.; Wells, D. J.

In: Gene Therapy, Vol. 8, No. 16, 2001, p. 1264-1270.

Research output: Contribution to journalArticle

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