Optimization of antibody labeling with rhenium-188 using a prelabeled MAG3 chelate

José L. Crudo, Martin M. Edreira, Esteban R. Obenaus, Marco Chinol, Giovanni Paganelli, Silvia G. De Castiglia

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We report the optimization of polyclonal IgG labeling by 188Re using S-benzoyl-MAG3 as a model for labeling monoclonal antibodies (MoAb). We examined the in vitro stability of the labeled protein and its localization and excretion in mice with induced focal inflammation. Stability in serum was greater than 85.5% after 24 h. Biodistribution and imaging studies following administration to mice showed mainly renal and hepatic excretion and high IT/NT ratios (4.5 and 4.6) at 24 and 48 h, respectively. This indirect method of labeling antibodies using a 188Re-labeled active ester of MAG3 produced 188Re-MAG3-IgG of high in vitro stability and favorable uptake at sites of focal inflammation.

Original languageEnglish
Pages (from-to)173-182
Number of pages10
JournalInternational Journal of Pharmaceutics
Volume248
Issue number1-2
DOIs
Publication statusPublished - Nov 6 2002

Fingerprint

Rhenium
Immunoglobulin G
Inflammation
Antibodies
Protein Stability
Esters
Monoclonal Antibodies
Serum
In Vitro Techniques
Renal Elimination
rhenium-mercaptoacetyltriglycine
Hepatobiliary Elimination
S-benzoylmercaptoacetyltriglycine

Keywords

  • Re-MAG
  • Re-MAG-IgG
  • Antibodies
  • Radiolabeling
  • Radiotherapy
  • Rhenium-188

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Optimization of antibody labeling with rhenium-188 using a prelabeled MAG3 chelate. / Crudo, José L.; Edreira, Martin M.; Obenaus, Esteban R.; Chinol, Marco; Paganelli, Giovanni; De Castiglia, Silvia G.

In: International Journal of Pharmaceutics, Vol. 248, No. 1-2, 06.11.2002, p. 173-182.

Research output: Contribution to journalArticle

Crudo, José L. ; Edreira, Martin M. ; Obenaus, Esteban R. ; Chinol, Marco ; Paganelli, Giovanni ; De Castiglia, Silvia G. / Optimization of antibody labeling with rhenium-188 using a prelabeled MAG3 chelate. In: International Journal of Pharmaceutics. 2002 ; Vol. 248, No. 1-2. pp. 173-182.
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