Optimization of antibody labeling with rhenium-188 using a prelabeled MAG3 chelate

José L. Crudo, Martin M. Edreira, Esteban R. Obenaus, Marco Chinol, Giovanni Paganelli, Silvia G. De Castiglia

Research output: Contribution to journalArticle

Abstract

We report the optimization of polyclonal IgG labeling by 188Re using S-benzoyl-MAG3 as a model for labeling monoclonal antibodies (MoAb). We examined the in vitro stability of the labeled protein and its localization and excretion in mice with induced focal inflammation. Stability in serum was greater than 85.5% after 24 h. Biodistribution and imaging studies following administration to mice showed mainly renal and hepatic excretion and high IT/NT ratios (4.5 and 4.6) at 24 and 48 h, respectively. This indirect method of labeling antibodies using a 188Re-labeled active ester of MAG3 produced 188Re-MAG3-IgG of high in vitro stability and favorable uptake at sites of focal inflammation.

Original languageEnglish
Pages (from-to)173-182
Number of pages10
JournalInternational Journal of Pharmaceutics
Volume248
Issue number1-2
DOIs
Publication statusPublished - Nov 6 2002

Keywords

  • Re-MAG
  • Re-MAG-IgG
  • Antibodies
  • Radiolabeling
  • Radiotherapy
  • Rhenium-188

ASJC Scopus subject areas

  • Pharmaceutical Science

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